Influence of Environmental pH on G2-Phase Arrest Caused by Ionizing Radiation

Autor: Heon Joo Park, Robert J. Griffin, Hyung Sik Lee, Yun Hee Rhee, Eun Kyung Choi, Sang Hwa Lee, HyunSook Chung, Sung Whan Ha, C. W. Song, Byung Uk Lim
Rok vydání: 2003
Předmět:
Zdroj: Radiation Research. 159:86-93
ISSN: 1938-5404
0033-7587
DOI: 10.1667/0033-7587(2003)159[0086:ioepog]2.0.co;2
Popis: Park, H. J., Lee, S. H., Chung, H., Rhee, Y. H., Lim, B. U., Ha, S. W., Griffin, R. J., Lee, H. S., Song, C. W. and Choi, E.;thK. Influence of Environmental pH on G2-Phase Arrest Caused by Ionizing Radiation. Radiat. Res. 159, 86–93 (2003). We investigated the effects of an acidic environment on the G2/M-phase arrest, apoptosis, clonogenic death, and changes in cyclin B1-CDC2 kinase activity caused by a 4-Gy irradiation in RKO.C human colorectal cancer cells in vitro. The time to reach peak G2/M-phase arrest after irradiation was delayed in pH 6.6 medium compared to that in pH 7.5 medium. Furthermore, the radiation-induced G2/M-phase arrest decayed more slowly in pH 6.6 medium than in pH 7.5 medium. Finally, there was less radiation-induced apoptosis and clonogenic cell death in pH 6.6 medium than in pH 7.5 medium. It appeared that the prolongation of G2-phase arrest after irradiation in the acidic environment allowed for greater repair of radiation-induced DNA damage, thereby decreasing the radi...
Databáze: OpenAIRE