Influence of Environmental pH on G2-Phase Arrest Caused by Ionizing Radiation
Autor: | Heon Joo Park, Robert J. Griffin, Hyung Sik Lee, Yun Hee Rhee, Eun Kyung Choi, Sang Hwa Lee, HyunSook Chung, Sung Whan Ha, C. W. Song, Byung Uk Lim |
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Rok vydání: | 2003 |
Předmět: |
G2 Phase
Cell Survival DNA damage Biophysics Cyclin B Ionizing radiation Toxicology Radiation Ionizing CDC2 Protein Kinase Tumor Cells Cultured Humans Radiology Nuclear Medicine and imaging Irradiation Cyclin B1 Phosphorylation Kinase activity Clonogenic assay Cyclin Radiation Chemistry Dose-Response Relationship Radiation Hydrogen-Ion Concentration Flow Cytometry Molecular biology In vitro Culture Media Apoptosis Colorectal Neoplasms |
Zdroj: | Radiation Research. 159:86-93 |
ISSN: | 1938-5404 0033-7587 |
DOI: | 10.1667/0033-7587(2003)159[0086:ioepog]2.0.co;2 |
Popis: | Park, H. J., Lee, S. H., Chung, H., Rhee, Y. H., Lim, B. U., Ha, S. W., Griffin, R. J., Lee, H. S., Song, C. W. and Choi, E.;thK. Influence of Environmental pH on G2-Phase Arrest Caused by Ionizing Radiation. Radiat. Res. 159, 86–93 (2003). We investigated the effects of an acidic environment on the G2/M-phase arrest, apoptosis, clonogenic death, and changes in cyclin B1-CDC2 kinase activity caused by a 4-Gy irradiation in RKO.C human colorectal cancer cells in vitro. The time to reach peak G2/M-phase arrest after irradiation was delayed in pH 6.6 medium compared to that in pH 7.5 medium. Furthermore, the radiation-induced G2/M-phase arrest decayed more slowly in pH 6.6 medium than in pH 7.5 medium. Finally, there was less radiation-induced apoptosis and clonogenic cell death in pH 6.6 medium than in pH 7.5 medium. It appeared that the prolongation of G2-phase arrest after irradiation in the acidic environment allowed for greater repair of radiation-induced DNA damage, thereby decreasing the radi... |
Databáze: | OpenAIRE |
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