Biohybrids of scaffolding hyaluronic acid biomaterials plus adipose stem cells home local neural stem and endothelial cells: Implications for reconstruction of brain lesions after stroke

Autor: Gonzalo León-Espinosa, Leyre Sanchez‐Rojas, M. Monleón Pradas, Juan A. Barcia, Cristina Lendinez, Fernando J. Rascón-Ramírez, María Soledad Benito-Martín, Cristina Martínez-Ramos, Jorge Matías-Guiu, Ulises Gómez-Pinedo
Přispěvatelé: Universidad Complutense de Madrid
Rok vydání: 2018
Předmět:
0301 basic medicine
Pathology
Adipose tissue
adiposestem cells
Biocompatible Materials
Cell therapy
chemistry.chemical_compound
0302 clinical medicine
Neural Stem Cells
Hyaluronic acid
Hyaluronic Acid
health care economics and organizations
Neurons
Tissue Scaffolds
Neurogenesis
Brain
Stroke
medicine.anatomical_structure
Adipose Tissue
MAQUINAS Y MOTORES TERMICOS
Models
Animal
Stem cell
Porosity
medicine.medical_specialty
Neurofilament
Materials science
Surface Properties
education
Biomedical Engineering
Mice
Nude
Subventricular zone
Biomaterials
03 medical and health sciences
Neuroblast
medicine
Animals
Humans
Cell Proliferation
Cell therapies
Tissue Engineering
Endothelial Cells
Thrombosis
Cerebral Arteries
Adipose stem cells
030104 developmental biology
chemistry
030217 neurology & neurosurgery
Zdroj: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
Digital.CSIC. Repositorio Institucional del CSIC
ISSN: 1552-4981
1552-4973
DOI: 10.1002/jbm.b.34252
Popis: Endogenous neurogenesis in stroke is insufficient to replace the lost brain tissue, largely due to the lack of a proper biological structure to let new cells dwell in the damaged area. We hypothesized that scaffolds made of hyaluronic acid (HA) biomaterials (BM) could provide a suitable environment to home not only new neurons, but also vessels, glia and neurofilaments. Further, the addition of exogenous cells, such as adipose stem cells (ASC) could increase this effect. Athymic mice were randomly assigned to a one of four group: stroke alone, stroke and implantation of BM, stroke and implantation of BM with ASC, and sham operated animals. Stroke model consisted of middle cerebral artery thrombosis with FeCl. After 30 days, animals underwent magnetic resonance imaging (MRI) and were sacrificed. Proliferation and neurogenesis increased at the subventricular zone ipsilateral to the ventricle and neuroblasts, glial, and endothelial cells forming capillaries were seen inside the BM. Those effects increased when ASC were added, while there was less inflammatory reaction. Three-dimensional scaffolds made of HA are able to home newly formed neurons, glia, and endothelial cells permitting the growth neurofilaments inside them. The addition of ASC increase these effects and decrease the inflammatory reaction to the implant. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1598–1606, 2019.
We thank for their skillful assistance at of the UniversidadComplutense de Madrid center: ICTS-Centro Nacional de Microscopía Electrónica for technical assistance in SEM; Microscopy and Flow Cytometry Center for confocal sup-port; NMR unit of the Bioimaging Center for technical assistance in MRI support. The authors thank Carolina Fuentes Suárez of U.P.R. for critical reading of the manuscript.
Databáze: OpenAIRE
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