Preparation of immobilized phosphodiesterase from calf spleen and its use in oligonucleotide analysis
| Autor: | E. H. Teufel, Manfred Philipp, H. Seliger |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
chemistry.chemical_classification
Phosphoric Diester Hydrolases Protein Conformation Chemistry Oligonucleotide Sepharose Spleen phosphodiesterase Oligonucleotides Phosphodiesterase Bioengineering Spleen Hydrogen-Ion Concentration Enzymes Immobilized Applied Microbiology and Biotechnology Enzyme medicine.anatomical_structure Biochemistry Ionic strength Concanavalin A Ph dependence medicine Animals Cattle Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 22:55-64 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.260220105 |
| Popis: | The phosphodiesterase from calf spleen (EC 3.1.4.18) was immobilized on several supports. Some properties of the most suitable enzyme support system--calf spleen phosphodiesterase bound to agarose-Concanavalin A--were investigated, e.g., pH dependence, influence of ionic strength of the buffer medium, and Zn2+-ion inhibition. The immobilized spleen phosphodiesterase showed about 60% of the activity of the free enzyme; the activity toward several oligonucleotide test substrates was unchanged for two months. |
| Databáze: | OpenAIRE |
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