Comparing glutamatergic neuron population in the mediodorsal thalamic nucleus of genetic absence epilepsy rats from strasbourg (GAERS) and normal control Wistar rats
| Autor: | Merve Özgür, Özlem Kirazlı, Sercin Karahuseyinoglu, Filiz Onat, Safiye Çavdar |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Mediodorsal Thalamic Nucleus Population Glutamic Acid Cell Count Biology 03 medical and health sciences Cellular and Molecular Neuroscience Glutamatergic Epilepsy 0302 clinical medicine Internal medicine medicine Animals Rats Wistar education Normal control Neurons education.field_of_study Glutamate receptor medicine.disease Immunohistochemistry Rats 030104 developmental biology medicine.anatomical_structure Endocrinology nervous system Epilepsy Absence Mediodorsal thalamic nucleus Neuron Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of chemical neuroanatomy. 77 |
| ISSN: | 1873-6300 |
| Popis: | An imbalance between GABAergic inhibition and glutamatergic excitation is suspected to play a role in the genesis of epileptic processes. In the present study we quantified the number of glutamate+ve neurons in the mediodorsal thalamic nucleus (MD) of genetic absence epilepsy rats from Strasbourg (GAERS) and compared these with values for normal Wistar rats. The MD thalamic nucleus was removed from each animal and the glutamatergic neurons were labelled using light-microscopy glutamate immunohistochemistry. The disector method was used to quantify the glutamate+ve neurons in the MD thalamic nucleus of GAERS and Wistar rats. The data were statistically analyzed. In the Wistar animals glutamate+ve neurons formed 89% and in GAERS 92.3% of the total neurons in 1000μm3 of MD thalamic nucleus. In GAERS glutamate+ve neurons showed statistically significant increase in the MD thalamic nucleus compared to Wistar animals. In Wistar animals the glutamate-ve neurons formed 11% and in GAERS 7.7% of the total neurons in 1000μm3 of MD thalamic. No significant difference was observed in glutamate-ve neurons between the two strains. The average diameter of glutamate+ve neurons showed no significance, while glutamate-ve neurons were significant between the two strains. The results of the present study, on genetic absence epilepsy model, GAERS, confirms the role of MD thalamic nucleus in chemically induced absence epilepsy. |
| Databáze: | OpenAIRE |
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