Phosphorylated epitopes of neurofilaments have been conserved during chordate evolution
| Autor: | Paolo Ruggiero, Mario Contorni, Caterina Mencarelli, Massimo Bugnoli, Vitaliano Pallini, A. Moscatelli |
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| Rok vydání: | 1987 |
| Předmět: |
Neurofilament
Protein subunit Intermediate Filaments Biophysics Chordate Biochemistry Epitope Epitopes Antibody Specificity Chordata Nonvertebrate Animals Phosphorylation Intermediate filament Cytoskeleton Molecular Biology biology Fishes Rana esculenta Cell Biology biology.organism_classification Biological Evolution Molecular biology Rats Polyclonal antibodies biology.protein Rabbits |
| Zdroj: | Biochemical and Biophysical Research Communications. 149:807-814 |
| ISSN: | 0006-291X |
| Popis: | We have characterized some rabbit polyclonal responses as strictly specific for phosphorylated epitopes located in the carboxyterminal (tail) domain of the H or the M subunits of mammalian neurofilaments. These antibodies have been used to confirm the occurrence in lizard neurofilaments of a single heavy subunit cross-reacting with both H and M from mammals. A heavy subunit with similar cross-reactivity has been detected in neurofilaments preparations from fishes, whereas more primitive Chordata possess a HMW polypeptide cross-reacting with only the M subunit. We could also demonstrate in frog spinal cord two distinct heavy subunits cross-reacting with either the M or the H subunit from mammals, a fact which suggests a convergent evolution for phosphorylated epitopes of neurofilaments. |
| Databáze: | OpenAIRE |
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