Molecular-imaging probe 2-(1-[6-[(2-fluoroethyl)(methyl) amino]-2-naphthyl]ethylidene) malononitrile labels prion plaques in vitro
| Autor: | Mara Popovic, Lojze M. Smid, Andrej Petric, Tomaz D. Vovko, Jorge R. Barrio, Mara Bresjanac |
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| Rok vydání: | 2003 |
| Předmět: |
Molecular Probe Techniques
Plaque Amyloid Sensitivity and Specificity Creutzfeldt-Jakob Syndrome Prion Diseases Substrate Specificity chemistry.chemical_compound In vivo Cerebellum mental disorders Nitriles medicine Gerstmann-Straussler-Scheinker Disease Senile plaques Fluoroethyl Malononitrile Fluorescent Dyes medicine.diagnostic_test Chemistry General Neuroscience Reproducibility of Results medicine.disease Fluorescence Immunohistochemistry In vitro nervous system diseases Biochemistry Positron emission tomography Molecular Probes Kuru Brief Communications |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 23(22) |
| ISSN: | 1529-2401 |
| Popis: | The study aimed to evaluate the fluorescent molecular-imaging probe 2-(1-{6-[(2-fluoroethyl)(methyl)amino]-2-naphthyl}ethylidene)malononitrile (FDDNP) for its ability to selectively and reproducibly label prion plaques in fixed, paraffin-embedded cerebellar sections from patients of confirmed Gerstmann-Sträussler-Scheinker disease, sporadic Creutzfeldt-Jacob disease (CJD) with kuru plaques, and variant CJD (vCJD). FDDNP is a highly hydrophobic, viscosity-sensitive, solvent-sensitive, fluorescent substance, whose radiofluorinated analog [18F]FDDNP has recently been successfully used to label senile plaques and neurofibrillary tangles in the living brain of Alzheimer's disease patients with positron emission tomography. Our results show that FDDNP reliably identifies all prion plaques, including small cluster-plaques in vCJD. This finding may open newin vivodiagnostic possibilities for vCJD. |
| Databáze: | OpenAIRE |
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