The changes in activation of intracellular aspartate aminotransferase by pyridoxal 5-phosphate after cell death
| Autor: | G. M. Potgieter, F.J. Burger |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
medicine.medical_specialty
Programmed cell death Clinical Biochemistry Stimulation Biochemistry Cofactor Liver disease chemistry.chemical_compound Internal medicine medicine Animals Aspartate Aminotransferases Pyridoxal chemistry.chemical_classification biology Myocardium Biochemistry (medical) General Medicine medicine.disease Molecular biology Rats Enzyme Activation Endocrinology Enzyme chemistry Liver Cytoplasm Postmortem Changes Pyridoxal Phosphate biology.protein Intracellular |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 84(1-2) |
| ISSN: | 0009-8981 |
| Popis: | Aspartate aminotransferase activity was measured in plasma, in liver and in heart mitochondrial and cytoplasmic preparations from rats immediately after death and after a post-mortem interval of 15 h. No significant stimulation of activity on addition of pyridoxal 5-phosphate to the assay medium could be demonstrated in any preparations obtained immediately after death. Significant stimulation occurred in both cytoplasmic and mitochondrial preparations of liver and myocardium after a 15-h post-mortem interval, but not in plasma stored for the same period. It appears, therefore, that variations in the intracellular saturation of apoenzyme with coenzyme cannot account for the observed differences in activation of aspartate aminotransferase by pyridoxal 5-phosphate in sera from patients with myocardial infarction and liver disease. Changes in degree of saturation of apoenzyme seem to occur intracellularly after cell death or injury and before release into the circulation. |
| Databáze: | OpenAIRE |
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