Adipose-Derived Stromal Cells Seeded on Integra® Dermal Regeneration Template Improve Post-Burn Wound Reconstruction
| Autor: | Agnieszka Waligórska, Justyna Drukala, Sylwia Bobis-Wozowicz, Eliza Zimoląg, Anna Chrapusta, Michał Nessler, Marcin Piejko, Karolina Radziun, Zbigniew Madeja |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell cell-based therapy Adipose tissue Scars wound healing Bioengineering Matrix metalloproteinase lcsh:Technology 03 medical and health sciences 0302 clinical medicine Tissue engineering Fibrosis medicine lcsh:QH301-705.5 030304 developmental biology 0303 health sciences lcsh:T Chemistry medicine.disease Transplantation lcsh:Biology (General) tissue engineering 030220 oncology & carcinogenesis burn-related scars medicine.symptom Wound healing collagen-based matrix |
| Zdroj: | Bioengineering Volume 7 Issue 3 Bioengineering, Vol 7, Iss 67, p 67 (2020) |
| ISSN: | 2306-5354 |
| DOI: | 10.3390/bioengineering7030067 |
| Popis: | Fibrosis of burn-related wounds remains an unresolved clinical issue that leads to patient disability. The aim of this study was to assess the efficacy of the transplantation of adipose-derived stromal cells seeded onto a collagen-based matrix in the reconstruction of burn-related scars. Here, we characterized an in vitro interaction between adipose-derived stromal cells and a collagen-based matrix, Integra® DRT. Our results show that transcription of pro-angiogenic, remodeling, and immunomodulatory factors was more significant in adipose-derived stromal cells than in fibroblasts. Transcription of metalloproteinases 2 and 9 is positively correlated with the collagenolytic activity of the adipose-derived stromal cells seeded onto Integra® DRT. The increase in the enzymatic activity corresponds to the decrease in the elasticity of the whole construct. Finally, we validated the treatment of a post-excision wound using adipose-derived stromal cells and an Integra® DRT construct in a 25-year-old woman suffering from burn-related scars. Scarless healing was observed in the area treated by adipose-derived stromal cells and the Integra® DRT construct but not in the reference area where Integra® DRT was applied without cells. This clinical observation may be explained by in vitro findings: Enhanced transcription of the vascular endothelial growth factor as well as remodeling of the collagen-based matrix decreased mechanical stress. Our experimental treatment demonstrated that the adipose-derived stromal cells seeded onto Integra® DRT exhibit valuable properties that may improve post-excision wound healing and facilitate skin regeneration without scars. |
| Databáze: | OpenAIRE |
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