The Effect of Corchorus olitorius L. Extract on Viability of Breast Cancer Cells: A friend or a foe?
| Autor: | Eda Becer, Günsu Soykut Çağsin, Seda Vatansever, Ihsan Calis |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Volume: 2, Issue: 2 113-118 Current Perspectives on Medicinal and Aromatic Plants (CUPMAP) |
| ISSN: | 2619-9645 2667-5722 |
| Popis: | This Corchorus olitorius L. isknown to be a medicinal plant widely consumed in the Eastern Mediterraneanregion and has anti-bacterial, anti-inflammatory and anti-cancer properties.Breast cancer has one of the highest mortality rates among all cancers.Therefore, the main aim of the study is to evaluate the effectiveness of C. olitorius plant on viability ofestrogen receptor (ER) positive breast cancer cell line, MCF-7. C. olitorius leaves first extracted withethanol, then LC-MS/MS analysis was done for identification of phytochemicals.MTT assay was used for assessment of cell viability of MCF-7 cells. Cells weretreated with C. olitorius extracts atfive concentrations (5, 10, 20, 50, 100 µg/ml) and four different incubationperiods (24, 48, 72, 96 h). LC-MS/MS analysis identified seven phytochemicalsin the extract, mainly quercetin and caffeoylquinic acid derivatives. MTTresults showed that the extract was only slightly effective in terms ofreduction of cell viability at 50 and 100 µg/ml doses which were incubated for24 and 48 h. Lower concentration doses did not show any effect in cellviability of MCF-7 cells. Longer incubation periods tend to increase cellviability of breast cancer cells. Quercetin identified within the extract might interfere with ER and promote MCF-7 cellproliferation. Therefore, in ER positive breast adenocarcinoma, quercetinintake and doses should carefully be monitored. More studies regarding therelationship between C. olitorius,quercetin and breast cancer should be done for further clarification of thetopic. |
| Databáze: | OpenAIRE |
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