Genetic diversity of Alternaria species associated with black point in wheat grains

Autor: Vladislav Shevtsov, Olesya Raiser, O.N. Khapilina, Ruslan Kalendar, Ainur Turzhanova, Asem Tumenbayeva
Přispěvatelé: Department of Agricultural Sciences
Rok vydání: 2020
Předmět:
0106 biological sciences
BARLEY
lcsh:Medicine
Molecular marker
01 natural sciences
Genetic diversity
chemistry.chemical_compound
REMAP
Genus
IRAP
ELEMENTS
Cultivar
11832 Microbiology and virology
2. Zero hunger
0303 health sciences
General Neuroscience
1184 Genetics
developmental biology
physiology
food and beverages
Alternaria
Biodiversity
General Medicine
DNA profiling
IPBS
POPULATIONS
General Agricultural and Biological Sciences
PHYLOGENY
Mycology
Biology
General Biochemistry
Genetics and Molecular Biology
4111 Agronomy
03 medical and health sciences
Botany
Genetics
otorhinolaryngologic diseases
Retrotransposon
Blight
Agricultural Science
030304 developmental biology
GENOME EVOLUTION
PATHOGENS
lcsh:R
fungi
Fungi
UPGMA
biology.organism_classification
chemistry
010606 plant biology & botany
Zdroj: PeerJ
PeerJ, Vol 8, p e9097 (2020)
ISSN: 2167-8359
Popis: The genus Alternaria is a widely distributed major plant pathogen that can act as a saprophyte in plant debris. Fungi of this genus frequently infect cereal crops and cause such diseases as black point and wheat leaf blight, which decrease the yield and quality of cereal products. A total of 25 Alternaria sp. isolates were collected from germ grains of various wheat cultivars from different geographic regions in Kazakhstan. We investigated the genetic relationships of the main Alternaria species related to black point disease of wheat in Kazakhstan, using the inter-primer binding site (iPBS) DNA profiling technique. We used 25 retrotransposon-based iPBS primers to identify the differences among and within Alternaria species populations, and analyzed the variation using clustering (UPGMA) and statistical approaches (AMOVA). Isolates of Alternaria species clustered into two main genetic groups, with species of A.alternata and A.tennuissima forming one cluster, and isolates of A. infectoria forming another. The genetic diversity found using retrotransposon profiles was strongly correlated with geographic data. Overall, the iPBS fingerprinting technique is highly informative and useful for the evaluation of genetic diversity and relationships of Alternaria species.
Databáze: OpenAIRE
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