Genetic diversity of Alternaria species associated with black point in wheat grains
Autor: | Vladislav Shevtsov, Olesya Raiser, O.N. Khapilina, Ruslan Kalendar, Ainur Turzhanova, Asem Tumenbayeva |
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Přispěvatelé: | Department of Agricultural Sciences |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
BARLEY lcsh:Medicine Molecular marker 01 natural sciences Genetic diversity chemistry.chemical_compound REMAP Genus IRAP ELEMENTS Cultivar 11832 Microbiology and virology 2. Zero hunger 0303 health sciences General Neuroscience 1184 Genetics developmental biology physiology food and beverages Alternaria Biodiversity General Medicine DNA profiling IPBS POPULATIONS General Agricultural and Biological Sciences PHYLOGENY Mycology Biology General Biochemistry Genetics and Molecular Biology 4111 Agronomy 03 medical and health sciences Botany Genetics otorhinolaryngologic diseases Retrotransposon Blight Agricultural Science 030304 developmental biology GENOME EVOLUTION PATHOGENS lcsh:R fungi Fungi UPGMA biology.organism_classification chemistry 010606 plant biology & botany |
Zdroj: | PeerJ PeerJ, Vol 8, p e9097 (2020) |
ISSN: | 2167-8359 |
DOI: | 10.7717/peerj.9097 |
Popis: | The genus Alternaria is a widely distributed major plant pathogen that can act as a saprophyte in plant debris. Fungi of this genus frequently infect cereal crops and cause such diseases as black point and wheat leaf blight, which decrease the yield and quality of cereal products. A total of 25 Alternaria sp. isolates were collected from germ grains of various wheat cultivars from different geographic regions in Kazakhstan. We investigated the genetic relationships of the main Alternaria species related to black point disease of wheat in Kazakhstan, using the inter-primer binding site (iPBS) DNA profiling technique. We used 25 retrotransposon-based iPBS primers to identify the differences among and within Alternaria species populations, and analyzed the variation using clustering (UPGMA) and statistical approaches (AMOVA). Isolates of Alternaria species clustered into two main genetic groups, with species of A.alternata and A.tennuissima forming one cluster, and isolates of A. infectoria forming another. The genetic diversity found using retrotransposon profiles was strongly correlated with geographic data. Overall, the iPBS fingerprinting technique is highly informative and useful for the evaluation of genetic diversity and relationships of Alternaria species. |
Databáze: | OpenAIRE |
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