Thermal Detection of Cardiac Biomarkers Heart-Fatty Acid Binding Protein and ST2 Using a Molecularly Imprinted Nanoparticle-Based Multiplex Sensor Platform
| Autor: | Robert D. Crapnell, Michael P. Down, Francesco Mecozzi, Marloes Peeters, Thomas J. Cleij, Francesco Canfarotta, Bart van Grinsven, Craig E. Banks, Richard Law, K. Betlem, Joanna Czulak, Rhiannon E Johnson, Kasper Eersels |
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| Přispěvatelé: | RS: FSE Sensor Engineering, Sensor Engineering, Faculty of Science and Engineering |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Cardiac biomarkers Nanoparticle Bioengineering 02 engineering and technology SOLID-PHASE SYNTHESIS Biosensing Techniques DIAGNOSIS 01 natural sciences RISK STRATIFICATION Molecular Imprinting heat-transfer method cardiac biomarkers SENSITIVITY TROPONIN-T Multiplex Surface plasmon resonance SOLUBLE ST2 Instrumentation Fluid Flow and Transfer Processes molecularly imprinted polymer nanoparticles (nanoMIPs) Process Chemistry and Technology 010401 analytical chemistry Molecularly imprinted polymer RECOGNITION heart-fatty acid binding protein (H-FABP) Surface Plasmon Resonance 021001 nanoscience & nanotechnology ST2 biosensors HFABP Interleukin-1 Receptor-Like 1 Protein 0104 chemical sciences Biomarker (cell) DERIVATION Heart-type fatty acid binding protein Nanoparticles thermal detection POLYMERS 0210 nano-technology Biosensor RULE Fatty Acid Binding Protein 3 Biomarkers Biomedical engineering |
| Zdroj: | ACS sensors, 4(10), 2838-2845. American Chemical Society |
| ISSN: | 2379-3694 |
| Popis: | This manuscript describes the production of molecularly imprinted polymer nanoparticles (nanoMIPs) for the cardiac biomarkers heart-fatty acid binding protein (H-FABP) and ST2 by solid-phase synthesis, and their use as synthetic antibodies in a multiplexed sensing platform. Analysis by surface plasmon resonance (SPR) shows that the affinity of the nanoMIPs is similar to that of commercially available antibodies. The particles are coated onto the surface of thermocouples and inserted into 3D-printed flow cells of different multiplexed designs. We demonstrate that it is possible to selectively detect both cardiac biomarkers within the physiologically relevant range. Furthermore, the developed sensor platform is the first example of a multiplex format of this thermal analysis technique which enables simultaneous measurements of two different compounds with minimal cross selectivity. The format where three thermocouples are positioned in parallel exhibits the highest sensitivity, which is explained by modeling the heat flow distribution within the flow cell. This design is used in further experiments and proof-of-application of the sensor platform is provided by measuring spiked fetal bovine serum samples. Because of the high selectivity, short measurement time, and low cost of this array format, it provides an interesting alternative to traditional immunoassays. The use of nanoMIPs enables a multimarker strategy, which has the potential to contribute to sustainable healthcare by improving the reliability of cardiac biomarker testing. |
| Databáze: | OpenAIRE |
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