An accumulation of muscle macrophages is accompanied by altered insulin sensitivity after reduced activity and recovery
| Autor: | Yu Kuei Lin, Micah J. Drummond, Paul T. Reidy, Matthew T. Rondina, Katie L Kaput, Jared H. Madsen, Nikol M. Yonemura, Ziad S. Mahmassani, Alec I. McKenzie |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Physiology Mrna expression Cell 030204 cardiovascular system & hematology Article 03 medical and health sciences 0302 clinical medicine Insulin resistance Immune system Internal medicine medicine Humans Insulin Muscle Skeletal Exercise Aged Aged 80 and over business.industry Macrophages Skeletal muscle Insulin sensitivity Middle Aged medicine.disease Capillaries 030104 developmental biology medicine.anatomical_structure Endocrinology Clamp Prospective clinical study Female Insulin Resistance Sedentary Behavior business |
| Zdroj: | Acta Physiol (Oxf) |
| ISSN: | 1748-1716 1748-1708 |
| DOI: | 10.1111/apha.13251 |
| Popis: | Mechanisms underlying physical inactivity-induced insulin resistance are not well understood. In addition to a role in muscle repair, immune cell populations such as macrophages may regulate insulin sensitivity. AIM. To examine if the dynamic changes in insulin sensitivity during and after recovery from reduced physical activity corresponded to changes in skeletal muscle macrophages. METHODS. In this prospective clinical study we collected muscle biopsies from healthy older adults (70±2y, n=12) before and during a hyperinsulinemic-euglycemic clamp were assessed before (PRE) and after 2-weeks reduced physical activity (RA), and following 2-weeks of recovery (REC). Insulin sensitivity (hyperinsulinemic-euglycemic clamp), skeletal muscle mRNA expression of inflammatory markers, and immunofluorescent quantification of skeletal muscle macrophages, myofiber-specific satellite cell and capillary content. RESULTS. Insulin sensitivity was decreased following reduced activity and rebounded following recovery above PRE levels. We observed an increase (p0.57, P |
| Databáze: | OpenAIRE |
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