(−)-Epigallocatechin-3-gallate inhibits Met signaling, proliferation, and invasiveness in human colon cancer cells
| Autor: | Christine A. Larsen, Roderick H. Dashwood |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Indoles Cell Survival Colorectal cancer medicine.medical_treatment Biophysics Antineoplastic Agents Epigallocatechin gallate Biology complex mixtures Biochemistry Catechin Piperazines Article Targeted therapy Metastasis Phosphatidylinositol 3-Kinases chemistry.chemical_compound Internal medicine medicine Humans Neoplasm Invasiveness Receptors Growth Factor Protein Kinase Inhibitors Molecular Biology Protein kinase B Cell Proliferation Sulfonamides Matrigel Tea Hepatocyte Growth Factor Cell growth food and beverages Proto-Oncogene Proteins c-met HCT116 Cells medicine.disease Endocrinology chemistry Colonic Neoplasms Cancer research Hepatocyte growth factor Signal Transduction medicine.drug |
| Zdroj: | Archives of Biochemistry and Biophysics. 501:52-57 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/j.abb.2010.03.017 |
| Popis: | The Met receptor tyrosine kinase is deregulated in a variety of cancers and is correlated with advanced stage and poor prognosis. Thus, Met has been identified as an attractive candidate for targeted therapy. We compared the tea polyphenol (−)-epigallocatechin-3-gallate (EGCG) and a specific Met inhibitor, SU11274, as suppressing agents of Met signaling in HCT116 human colon cancer cells. Treatment with hepatocyte growth factor increased phospho-Met levels, and this was inhibited in a concentration-dependent manner by EGCG and SU11274 (IC 50 3.0 vs. 0.05 μM, respectively). Downstream activation of Erk and Akt signaling pathways also was suppressed. Both compounds at a concentration of 5 μM lowered cell viability and proliferation, with EGCG being more effective than SU11274, and the invasion of colon cancer cells in Matrigel assays was strongly inhibited. These findings are discussed in the context of the pleiotropic effects of tea catechins, their tissue metabolite levels, and the potential to inhibit colon cancer metastasis and invasion. |
| Databáze: | OpenAIRE |
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