Differential Metabolic and Transcriptional Responses of Gilthead Seabream (Sparus aurata) Administered with Cortisol or Cortisol-BSA
| Autor: | R. Oyarzún, Ignacio Ruiz-Jarabo, Jorge E. Aedo, Daniela Aravena-Canales, Juan Miguel Mancera, Gonzalo Martínez-Rodríguez, Alfredo Molina, Juan Antonio Valdés |
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| Přispěvatelé: | Biología, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (Chile), Fondo Nacional de Desarrollo Científico y Tecnológico (Chile), Ministerio de Economía y Competitividad (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Gilthead Seabream endocrine system energetic metabolism food.ingredient Veterinary medicine Cell Carbohydrate metabolism cortisol Article food Sparus aurata Internal medicine SF600-1100 Gene expression medicine Fisiología animal fish General Veterinary glucocorticoids Chemistry Coconut oil Skeletal muscle stress response medicine.anatomical_structure Endocrinology QL1-991 Peces Fish Animal Science and Zoology Zoology hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Animals Volume 11 Issue 11 Animals 2021, 11(11), 3310 RODIN. Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz instname E-Prints Complutense. Archivo Institucional de la UCM Animals : an Open Access Journal from MDPI Animals, Vol 11, Iss 3310, p 3310 (2021) |
| ISSN: | 2076-2615 |
| DOI: | 10.3390/ani11113310 |
| Popis: | Cortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of this hormone during short (minutes to hours)- and long-term (days to weeks) responses to stress. The role of membrane-initiated cortisol signaling during long-term responses has been recently explored. In this study, Sparus aurata were intraperitoneally injected with coconut oil alone or coconut oil containing cortisol, cortisol-BSA, or BSA. After 3 days of treatment, plasma, liver, and skeletal muscle were extracted. Plasma cortisol, as well as metabolic indicators in the plasma and tissues collected, and metabolism-related gene expression, were measured. Our results showed that artificially increased plasma cortisol levels in S. aurata enhanced plasma glucose and triacylglycerols values as well as hepatic substrate energy mobilization. Additionally, cortisol stimulated hepatic carbohydrates metabolism, as seen by the increased expression of metabolism-related genes. All of these responses, observed in cortisol-administered fish, were not detected by replicating the same protocol and instead using cortisol-BSA, which exclusively induces membrane-initiated effects. Therefore, we suggest that after three days of cortisol administration, only genomic actions are involved in the metabolic responses in S. aurata. This work was funded by Spanish Ministry of Science and Innovation—MICINN (PID2020-117557RB-C22) awarded to JMM; Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (FONDAP) Grant INCAR 15110027; Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) Grant 1201498 (to Juan Antonio Valdés), and ANID FONDECYT Postdoctorado [grant number 3210050] awarded to Jorge E. Aedo. The authors (I.R, G.M, and J.M.M) belong to the Fish Welfare and Stress Network (AGL2016-81808-REDT), supported by the Agencia Estatal de Investigación (MICINN, Spanish Government). |
| Databáze: | OpenAIRE |
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