Food Protein Based Core–Shell Nanocarriers for Oral Drug Delivery: Effect of Shell Composition on in Vitro and in Vivo Functional Performance of Zein Nanocarriers
| Autor: | Mohammed S. Alqahtani, M. Saiful Islam, Satheesh Podaralla, Joshua Reineke, Tofuko A Woyengo, Omathanu Perumal, Radhey S. Kaushik |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Chemistry Pharmaceutical Zein Dispersity Pharmaceutical Science Nanoparticle Nanotechnology 02 engineering and technology 030226 pharmacology & pharmacy Micelle Polyethylene Glycols Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems 0302 clinical medicine In vivo Cell Line Tumor Drug Discovery Animals Humans Tissue Distribution Particle Size Micelles Drug Carriers Mice Inbred BALB C Chemistry Nile red Proteins 021001 nanoscience & nanotechnology Rats Food Ionic strength Biophysics Nanoparticles Molecular Medicine Female Particle size Caco-2 Cells Nanocarriers 0210 nano-technology Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Molecular Pharmaceutics. 14:757-769 |
| ISSN: | 1543-8392 1543-8384 |
| DOI: | 10.1021/acs.molpharmaceut.6b01017 |
| Popis: | The study was aimed at systematically investigating the influence of shell composition on the particle size, stability, release, cell uptake, permeability, and in vivo gastrointestinal distribution of food protein based nanocarriers for oral delivery applications. Three different core-shell nanocarriers were prepared using food-grade biopolymers including zein-casein (ZC) nanoparticles, zein-lactoferrin (ZLF), nanoparticles and zein-PEG (ZPEG) micelles. Nile red was used as a model hydrophobic dye for in vitro studies. The nanocarriers had negative, positive, and neutral charge, respectively. All three nanocarriers had a particle size of less than 200 nm and a low polydispersity index. The nanoparticles were stable at gastrointestinal pH (2-9) and ionic strength (10-200 mM). The nanocarriers sustained the release of Nile red in simulated gastric and intestinal fluids. ZC nanoparticles showed the slowest release followed by ZLF nanoparticles and ZPEG micelles. The nanocarriers were taken up by endocytosis in Caco-2 cells. ZPEG micelles showed the highest cell uptake and transepithelial permeability followed by ZLF and ZC nanoparticles. ZPEG micelles also showed P-gp inhibitory activity. All three nanocarriers showed bioadhesive properties. Cy 5.5, a near IR dye, was used to study the in vivo biodistribution of the nanocarriers. The nanocarriers showed longer retention in the rat gastrointestinal tract compared to the free dye. Among the three formulations, ZC nanoparticles was retained the longest in the rat gastrointestinal tract (≥24 h). Overall, the outcomes from this study demonstrate the structure-function relationship of core-shell protein nanocarriers. The findings from this study can be used to develop food protein based oral drug delivery systems with specific functional attributes. |
| Databáze: | OpenAIRE |
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