A cell-based sensor system for toxicity testing using multiwavelength fluorescence spectroscopy
| Autor: | Michael Fritzsche, Maria Carlsson, Carl-Fredrik Mandenius, J. Magnus Fredriksson |
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| Rok vydání: | 2009 |
| Předmět: |
Chemistry
Biophysics In vitro toxicology Analytical chemistry Reproducibility of Results Cell Biology Biochemistry Fluorescence In vitro Fluorescence spectroscopy Matrix (chemical analysis) Mice Autofluorescence Microtiter plate Spectrometry Fluorescence In vivo Toxicity Tests Animals Myocytes Cardiac Molecular Biology Cells Cultured Biomedical engineering |
| Zdroj: | Analytical Biochemistry. 387:271-275 |
| ISSN: | 0003-2697 |
| Popis: | A novel cell-based fluorometric sensor system for toxicity monitoring is described, which uses functional spontaneously contracting cardiomyocytes (HL-1 cell line) as the biological recognition element. Based on these highly specialized cells, it has the potential of providing a sensitive and relevant analytical in vitro toxicity testing method. The system was configured by propagating the surface-attaching HL-1 cardiomyocytes in the wells of a 96-well microtiter plate and connecting the plate via an optical fiber to a fluorescence spectrometer capable of excitation-emission matrix scanning. The fluorescence data were analyzed using a conventional spectral analysis software program. The performance of the system for detection of general cytotoxicity to the cells was evaluated using three well-known drugs: verapamil, quinidine, and acetaminophen. The dose-response curves were assessed and the EC50 values were determined (0.10 +/- 0.007, 0.23 +/- 0.025, and 12.32 +/- 2.40 mM, respectively). Comparison with in vitro and in vivo reference data for the drugs showed good correlations, suggesting that this cell-based sensor system Could be a useful tool in pharmacological in vitro drug testing. |
| Databáze: | OpenAIRE |
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