Achieving Interconnected Pore Architecture in Injectable PolyHIPEs for Bone Tissue Engineering
| Autor: | Madison A.P. McEnery, Jennifer L. Robinson, Melissa C. Stuebben, Elizabeth Cosgriff-Hernandez, Robert S. Moglia |
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| Rok vydání: | 2014 |
| Předmět: |
Time Factors
Materials science Compressive Strength Cell Survival Polymers Sus scrofa Biomedical Engineering Bioengineering Polypropylenes Biochemistry Bone and Bones Injections Styrenes Biomaterials Fumarates Tissue engineering Elastic Modulus medicine Animals Humans Porosity chemistry.chemical_classification Tissue Engineering Viscosity Mesenchymal Stem Cells Original Articles Polymer Compressive strength medicine.anatomical_structure Polymerization chemistry Emulsion Microscopy Electron Scanning Radical initiator Cancellous bone Biomedical engineering |
| Zdroj: | Tissue Engineering Part A. 20:1103-1112 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2013.0319 |
| Popis: | Template polymerization of a high internal phase emulsion (polyHIPE) is a relatively new method to produce tunable high-porosity scaffolds for tissue regeneration. This study focuses on the development of biodegradable injectable polyHIPEs with interconnected porosity that have the potential to fill bone defects and enhance healing. Our laboratory previously fabricated biodegradable polyHIPEs that cure in situ upon injection; however, these scaffolds possessed a closed-pore morphology, which could limit bone ingrowth. To address this issue, HIPEs were fabricated with a radical initiator dissolved in the organic phase rather than the aqueous phase of the emulsion. Organic-phase initiation resulted in macromer densification forces that facilitated pore opening during cure. Compressive modulus and strength of the polyHIPEs were found to increase over 2 weeks to 43±12 MPa and 3±0.2 MPa, respectively, properties comparable to cancellous bone. The viscosity of the HIPE before cure (11.0±2.3 Pa·s) allowed for injection and filling of the bone defect, retention at the defect site during cure under water, and microscale integration of the graft with the bone. Precuring the materials before injection allowed for tuning of the work and set times. Furthermore, storage of the HIPEs before cure for 1 week at 4°C had a negligible effect on pore architecture after injection and cure. These findings indicate the potential of these emulsions to be stored at reduced temperatures and thawed in the surgical suite before injection. Overall, this work highlights the potential of interconnected propylene fumarate dimethacrylate polyHIPEs as injectable scaffolds for bone tissue engineering. |
| Databáze: | OpenAIRE |
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