Highly-efficient, fluorescent, locus directed cre and FlpO deleter mice on a pure C57BL/6N genetic background
| Autor: | Andrée Dierich, Sylvie Jacquot, Yann Herault, Marie-Christine Birling, Guillaume Pavlovic |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
RNA
Untranslated Genotype Transgene Green Fluorescent Proteins Mice Transgenic Locus (genetics) Biology Green fluorescent protein Mice 03 medical and health sciences 0302 clinical medicine Endocrinology Bacterial Proteins Genetics Recombinase Animals Allele Homologous Recombination Sequence Deletion 030304 developmental biology 0303 health sciences Integrases Wild type Proteins Gene targeting Cell Biology Molecular biology Founder Effect Mice Inbred C57BL Luminescent Proteins DNA Nucleotidyltransferases Gene Targeting Genetic Engineering Homologous recombination 030217 neurology & neurosurgery |
| Zdroj: | genesis; Vol 50 genesis |
| ISSN: | 1526-954X |
| DOI: | 10.1002/dvg.20826 |
| Popis: | To facilitate the use of the new mutant resource developed in the mouse, we have generated Cre and FlpO deleter mice on a pure inbred C57BL/6N background. The new transgenic constructs were designed to drive either the Cre or FlpO recombinase, fused to a specific fluorescent marker, respectively the eGFP or the eYFP, and were inserted by homologous recombination in the neutral Rosa26 locus. They allow a rapid, cost-effective, and efficient identification of the carrier individuals through the coexpression of the fluorescent marker. The recombination efficiency of the two deleter lines, Gt(ROSA)26S ortm1(ACTB-cre,-EGFP)Icsand Gt(ROSA) 26S ortm2(CAG-flpo, EYFP)Ics, was carefully evaluated using five loxP-flanked or four FRT-flanked alleles located at different positions in the mouse genome. For each tested locus, we observed a 100% excision rate. The transgenic mice are easily distinguishable from wild type animals by their bright fluorescence that remains easily detectable until 10 days after birth. In the adult, fluorescence can still be detected in the unpigmented paws. Furthermore, they both display accumulation of the specific recombinase during oogenesis. These fluorescent 'Cre- and Flp- deleter' transgenic lines are valuable tools for the scientific community by their high and stable recombination efficiency, the simplicity of genotype identification and the maintenance of a pure genetic background when used to remove specific selection cassette or to induce complete loss-of-function allele. |
| Databáze: | OpenAIRE |
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