Genotoxic and Inflammatory Effects of Depleted Uranium Particles Inhaled by Rats

Autor: Marjorie Monleau, Gérard Duménil, V. Chazel, Michel De Méo, François Paquet, Marie Donnadieu-Claraz
Přispěvatelé: Laboratoire de radiotoxicologie et radiobiologie expérimentale (IRSN/PSE-SANTE/SESANE/LRTOX), Service de recherche sur les effets biologiques et Sanitaires des rayonnements ionisants (IRSN/PSE-SANTE/SESANE), Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Université de la Méditerranée - Aix-Marseille 2, Laboratoire de radiotoxicologie et radiobiologie expérimentale (LRTOX), Institut de Radioprotection et de Sûreté Nucléaire (IRSN)
Rok vydání: 2005
Předmět:
Male
[SDV]Life Sciences [q-bio]
Gene Expression
radiation exposure
animal cell
010501 environmental sciences
Pharmacology
Kidney
Toxicology
medicine.disease_cause
Bronchoalveolar Lavage
01 natural sciences
Rats
Sprague-Dawley
Fibrosis
cytokine
kidney cell
oxidative stress
rat
correlation function
lung alveolus cell
DNA strand breakage
Inhalation Exposure
0303 health sciences
Inhalation
Chemistry
nephrotoxicity
article
Specific Pathogen-Free Organisms
3. Good health
hydroperoxide
Uranium
Cytokines
Comet Assay
medicine.symptom
radiation dose
Bronchoalveolar Lavage Fluid
Radioactive Pollutants
DNA damage
animal experiment
Industrial Waste
Inflammation
Respiratory Mucosa
animal tissue
in vivo study
03 medical and health sciences
In vivo
medicine
Animalia
Animals
controlled study
protein expression
030304 developmental biology
0105 earth and related environmental sciences
lung toxicity
nonhuman
animal model
solubility
genotoxicity
uranium dioxide
Epithelial Cells
DNA
Hydrogen Peroxide
medicine.disease
Rats
Comet assay
inflammation
13. Climate action
Immunology
Sprague-Dawley
Oxidative stress
Genotoxicity
Mutagens
Zdroj: Toxicological Sciences
Toxicological Sciences, 2006, 89 (1), pp.287-295. ⟨10.1093/toxsci/kfj010⟩
TOXICOLOGICAL SCIENCES
TOXICOLOGICAL SCIENCES, 2006, 89 (1), pp.287-295. ⟨10.1093/toxsci/kfj010⟩
ISSN: 1096-0929
1096-6080
1096-6099
DOI: 10.1093/toxsci/kfj010
Popis: Depleted uranium (DU) is a radioactive heavy metal coming from the nuclear industry and used in numerous military applications. Uranium inhalation can lead to the development of fibrosis and neoplasia in the lungs. As little is known concerning the molecular processes leading to these pathological effects, some of the events in terms of genotoxicity and inflammation were investigated in rats exposed to DU by inhalation. Our results show that exposure to DU by inhalation resulted in DNA strand breaks in broncho-alveolar lavage (BAL) cells and in increase of inflammatory cytokine expression and production of hydroperoxides in lung tissue suggesting that the DNA damage was in part a consequence of the inflammatory processes and oxidative stress. The effects seemed to be linked to the doses, were independent of the solubility of uranium compounds and correlating with the type of inhalation. Repeated inhalations seemed to induce an effect of potentiation in BAL cells and also in kidney cells. Comet assay in neutral conditions revealed that DNA damage in BAL cells was composed partly by double strands breaks suggesting that radiation could contribute to DU genotoxic effects in vivo. All these in vivo results contribute to a better understanding of the pathological effect of DU inhalation. © The Author 2005. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.
Databáze: OpenAIRE
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