Isolation and characterisation of potential respiratory syncytial virus receptor(s) on epithelial cells
| Autor: | Rajneesh Malhotra, Michael I. Bird, Michael Hurle, Malcolm Ward, Richard Priest, Martyn R. Foster, Eddie Blair, Helen Bright |
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| Rok vydání: | 2003 |
| Předmět: |
Paramyxoviridae
viruses Immunology Respiratory Syncytial Virus Infections Biology Microbiology Cell Line Mice Viral Proteins Annexin Polysaccharides Animals Humans L-Selectin Mononegavirales Receptor Annexin A2 Mice Inbred BALB C Virus receptor Biphenyl Compounds virus diseases Epithelial Cells respiratory system biology.organism_classification Virology Infectious Diseases Cell culture Mannosides Respiratory Syncytial Virus Human Receptors Virus Mannose Selectin |
| Zdroj: | Microbes and infection. 5(2) |
| ISSN: | 1286-4579 |
| Popis: | Respiratory syncytial virus (RSV) infection causes severe lower respiratory diseases in infancy, early childhood and the elderly. RSV infections respond poorly to current therapies. Therefore, we initiated a search for novel drug targets by investigating the characteristics and identity of RSV adhesion receptors on mammalian cells. Soluble human lectins, complex polysaccharides and a low molecular selectin antagonist, TBC1269, were used to characterise and isolate the RSV receptor on a human epithelial cell line (Hep2 cells). The binding characteristics of the RSV receptor on Hep2 cells were similar to those reported for L-selectin. The carbohydrate-based selectin antagonists, fucoidan and TBC 1269, inhibit RSV infection both in vitro and in a mouse model of infection. Furthermore, we have isolated annexin II as a potential RSV receptor on Hep2 cells. The expression of annexin II was increased after RSV infection. Recombinant annexin II binds to RSV G-protein, heparin and plasminogen and the binding is inhibited by a selectin antagonist, TBC1269. These findings indicate that inhibitors of annexin II could have potential in treating RSV infection. |
| Databáze: | OpenAIRE |
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