Expression of Vascular Endothelial Growth Factor Has a Regulatory Role in Gingival Venules in Experimental Diabetes
| Autor: | László Rosivall, Milán Gyurkovics, Izabella Nagy, Andrea Székely, Elek Dinya, Csaba Bödör, Zsolt Lohinai |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A 0301 basic medicine medicine.medical_specialty Gingiva Blood sugar Vasodilation Diabetes Mellitus Experimental 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Venules Diabetes mellitus Internal medicine medicine Animals Rats Wistar Venule business.industry 030206 dentistry medicine.disease Streptozotocin Rats Vascular endothelial growth factor Vascular endothelial growth factor A 030104 developmental biology Endocrinology chemistry Immunology Periodontics Immunohistochemistry business medicine.drug |
| Zdroj: | Journal of Periodontology. 87:e27-e34 |
| ISSN: | 1943-3670 0022-3492 |
| DOI: | 10.1902/jop.2015.150410 |
| Popis: | In the present study, the possible localization and role of vascular endothelial growth factor receptor type 2 (VEGFR2) in the regulation of gingival venules in a rat model of experimental diabetes are examined.Six weeks after streptozotocin premedication, Wistar male rats presenting blood sugar levels20 mmol/L were selected for investigation. The VEGFR2 antagonist ZM323881 [5-((7-benzyloxyquinazolin-4-yl)amino)-4-fluoro-2-methylphenol-hydrochloride] (20 μg/mL) was dripped onto the gingiva between the mandibular incisors. Changes in diameter of the selected gingival venule were measured by vital microscopy combined with digital photography at specified times. Immunohistochemical staining was used to localize VEGFR2. For controls, the same protocol was used on animals with normal blood sugar levels and healthy gingiva.There was a significant difference between the baseline venule diameter of the diabetic and the control groups (47 ± 1 and 28 ± 2 μm, respectively). After 15, 30, and 60 minutes of local application of ZM323881, significant vasoconstriction was observed in the venules of diabetic rats compared with the baseline (81.4% ± 4.6%, 81.8% ± 4.4%, and 80.6% ± 5.1%, respectively). The control group showed no change in the venule diameter. The immunohistochemical analysis showed significantly increased VEGFR2 expression in the mast cells along the venules in the diabetic group, whereas mast cells were rarely found in the control group.The findings suggest that VEGF expression is increased in gingiva in experimentally induced diabetes. After VEGFR2 activation, the mast cell-derived vasodilatory and inflammatory mediators may contribute markedly to the concomitant changes in the microcirculation. |
| Databáze: | OpenAIRE |
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