The mechanism of the G0/G1 cell cycle phase arrest induced by activation of PXR in human cells
| Autor: | Yanghao Guo, Jun Chen, Zhenhua Jia, Hang Wang, Chun Meng, Huahua Feng, Weiliang Zhuang |
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| Rok vydání: | 2010 |
| Předmět: |
Receptors
Steroid Transcription Genetic Ultraviolet Rays Recombinant Fusion Proteins Cell Biology Real-Time Polymerase Chain Reaction Binding Competitive Resting Phase Cell Cycle Cell cycle phase Neuroblastoma Cell Line Tumor medicine Humans RNA Small Interfering Pharmacology Pregnane X receptor Retinoid X Receptor alpha Cell growth G1 Phase Pregnane X Receptor General Medicine Hep G2 Cells Cell cycle G1 Phase Cell Cycle Checkpoints digestive system diseases Cell biology medicine.anatomical_structure Cell culture Cell cycle phase transition RNA Interference Rifampin G1 phase Cell Division DNA Damage |
| Zdroj: | Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 65(7) |
| ISSN: | 1950-6007 |
| Popis: | Context Pregnane X receptor (PXR) is an important transcriptional regulator that plays important roles in the cell metabolism and cell growth by regulating the transcriptional of a sort of metabolizing enzymes. Objective To investigate whether rifampicin effected HepG2 cells growth and the inhibition was due to the G0/G1 phase arrest. Methods PXR-knockdown experiments using RNAi showed that the cell cycle phase arrest mediated by rifampicin based on activation of PXR. The results also indicated that cell phase arrest by rifampicin could protect cells form UVB-induced DNA damage. Retinoid X receptor alpha (RXRα) expression level in cells is another key factor for cell cycle phase arrest mediated by rifampicin. Both over expression and lacking expression of RXRα in cell reduced the cell arrest efficiency mediated by rifampicin. In the study, we found that rifampicin inhibited HepG2 cells growth and demonstrated that the inhibition is due to the G0/G1 phase arrest through flow cytometry analysis. Conclusion The results showed that RXRα promote cell cycle phase transition rate of HepG2. Competitive bind of rifampicin-activated PXR with RXRα is one main reason to arrest cell cycle phase through inhibiting combination of RXRα with other partners. Rifampicin could promote cell growth rate when RXRα expressed more excessively than PXR in cells. |
| Databáze: | OpenAIRE |
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