Separation of ctDNA by superparamagnetic bead particles in microfluidic platform for early cancer detection
| Autor: | Mohd Ridzuan Ahmad, Samla Gauri Balakrishnan, Seyed Saeid Rahimian Koloor, Michal Petrů |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Medicine (General) Science (General) Microfluidics Circulating Tumor DNA 03 medical and health sciences Q1-390 0302 clinical medicine R5-920 Neoplasms medicine Circulating tumor DNA (ctDNA) detection Humans Sample preparation Early Cancer Detection Liquid biopsy ComputingMethodologies_COMPUTERGRAPHICS COMSOL Multiphysics Simulation study Multidisciplinary Cancer biomarker Chemistry Extraction (chemistry) Liquid Biopsy Cancer medicine.disease 030104 developmental biology Mathematics Engineering and Computer Science 030220 oncology & carcinogenesis Magnetic Iron Oxide Nanoparticles Particle size Liquid biopsy Magnetic manipulation Biomedical engineering Superparamagnetism |
| Zdroj: | Journal of Advanced Research, Vol 33, Iss, Pp 109-116 (2021) Journal of Advanced Research |
| ISSN: | 2090-1232 |
| Popis: | Graphical abstract Highlights • CtDNA extraction from blood samples by superparamagnetic (SPM) bead particles in a microfluidic platform for early cancer detection was proposed. • Computer experiments were conducted in COMSOL Multiphysics 5.3a software by using blood samples from stage I and II cancer patients to extract ctDNA. • An average of 5.7 ng of ctDNA was separated for every 10 µL of whole plasma input. • The simulation also showed that the device has ctDNA detection sensitivity and specificity of 65.57% and 95.38%, respectively for samples from stage I and II cancer patients. • The microchannel design with filtration method and separation by SPM bead particles has proved the effective ctDNA extraction from samples of stage I and II cancer patients. Introduction Conventional biopsy, based on extraction from a tumor of a solid tissue specimen requiring needles, endoscopic devices, excision or surgery, is at risk of infection, internal bleeding or prolonged recovery. A non-invasive liquid biopsy is one of the greatest axiomatic consequences of the identification of circulating tumor DNA (ctDNA) as a replaceable surgical tumor bioQpsy technique. Most of the literature studies thus far presented ctDNA detection at almost final stage III or IV of cancer, where the treatment option or cancer management is nearly impossible for diagnosis. Objective Hence, this paper aims to present a simulation study of extraction and separation of ctDNA from the blood plasma of cancer patients of stage I and II by superparamagnetic (SPM) bead particles in a microfluidic platform for early and effective cancer detection. Method The extraction of ctDNA is based on microfiltration of particle size to filter some impurities and thrombocytes plasma, while the separation of ctDNA is based on magnetic manipulation to high yield that can be used for the upstream process. Result Based on the simulation results, an average of 5.7 ng of ctDNA was separated efficiently for every 10 µL blood plasma input and this can be used for early analysis of cancer management. The particle tracing module from COMSOL Multiphysics traced ctDNA with 65.57% of sensitivity and 95.38% of specificity. Conclusion The findings demonstrate the ease of use and versatility of a microfluidics platform and SPM bead particles in clinical research related to the preparation of biological samples. As a sample preparation stage for early analysis and cancer diagnosis, the extraction and separation of ctDNA is most important, so precision medicine can be administered. |
| Databáze: | OpenAIRE |
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