Myosin II Motors and F-Actin Dynamics Drive the Coordinated Movement of the Centrosome and Soma during CNS Glial-Guided Neuronal Migration
| Autor: | Shaun S. Gleason, Niraj Trivedi, Eve-Ellen Govek, Mary E. Hatten, Ryan A. Kerekes, David J. Solecki |
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| Rok vydání: | 2009 |
| Předmět: |
Neuroscience(all)
Green Fluorescent Proteins Cell Cycle Proteins macromolecular substances Biology Transfection MOLNEURO Article Mice 03 medical and health sciences 0302 clinical medicine Cell Movement Tubulin Cerebellum Cell polarity Myosin medicine Animals Humans RNA Small Interfering Cytoskeleton Cells Cultured Actin Adaptor Proteins Signal Transducing 030304 developmental biology Centrosome Myosin Type II Neurons rho-Associated Kinases 0303 health sciences General Neuroscience Cell Polarity Membrane Proteins Fluorescence recovery after photobleaching Actins Cell biology Transport protein Protein Transport medicine.anatomical_structure Animals Newborn CELLBIO Soma Neuroglia 030217 neurology & neurosurgery Fluorescence Recovery After Photobleaching |
| Zdroj: | Neuron. 63(1):63-80 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2009.05.028 |
| Popis: | Lamination of cortical regions of the vertebrate brain depends on glial-guided neuronal migration. The conserved polarity protein Par6alpha localizes to the centrosome and coordinates forward movement of the centrosome and soma in migrating neurons. The cytoskeletal components that produce this unique form of cell polarity and their relationship to polarity signaling cascades are unknown. We show that F-actin and Myosin II motors are enriched in the neuronal leading process and that Myosin II activity is necessary for leading process actin dynamics. Inhibition of Myosin II decreased the speed of centrosome and somal movement, whereas Myosin II activation increased coordinated movement. Ectopic expression or silencing of Par6alpha inhibited Myosin II motors by decreasing Myosin light-chain phosphorylation. These findings suggest leading-process Myosin II may function to "pull" the centrosome and soma forward during glial-guided migration by a mechanism involving the conserved polarity protein Par6alpha. |
| Databáze: | OpenAIRE |
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