Nanoparticle based bio-bar code technology for trace analysis of aflatoxin B1 in Chinese herbs
| Autor: | Yuan-yuan Chen, Yu-yan Yu, Hong-yan Zhang, Tong-Ying Wang, Gao Xuan, Yuan-yuan Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Aflatoxin
Aflatoxin B1 medicine.drug_class Metal Nanoparticles Nanoparticle Enzyme-Linked Immunosorbent Assay lcsh:TX341-641 02 engineering and technology Monoclonal antibody Polymerase Chain Reaction 01 natural sciences chemistry.chemical_compound medicine Pharmacology Electronic Data Processing Chromatography biology Oligonucleotide lcsh:RM1-950 010401 analytical chemistry 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences lcsh:Therapeutics. Pharmacology Real-time polymerase chain reaction chemistry Polyclonal antibodies biology.protein Gold Drug Contamination 0210 nano-technology lcsh:Nutrition. Foods and food supply DNA Drugs Chinese Herbal Food Science |
| Zdroj: | Journal of Food and Drug Analysis, Vol 26, Iss 2, Pp 815-822 (2018) |
| ISSN: | 1021-9498 |
| DOI: | 10.1016/j.jfda.2017.11.003 |
| Popis: | A novel and sensitive assay for aflatoxin B1 (AFB1) detection has been developed by using bio-bar code assay (BCA). The method that relies on polyclonal antibodies encoded with DNA modified gold nanoparticle (NP) and monoclonal antibodies modified magnetic microparticle (MMP), and subsequent detection of amplified target in the form of bio-bar code using a fluorescent quantitative polymerase chain reaction (FQ-PCR) detection method. First, NP probes encoded with DNA that was unique to AFB1, MMP probes with monoclonal antibodies that bind AFB1 specifically were prepared. Then, the MMP-AFB1-NP sandwich compounds were acquired, dehybridization of the oligonucleotides on the nanoparticle surface allows the determination of the presence of AFB1 by identifying the oligonucleotide sequence released from the NP through FQ-PCR detection. The bio-bar code techniques system for detecting AFB1 was established, and the sensitivity limit was about 10−8 ng/mL, comparable ELISA assays for detecting the same target, it showed that we can detect AFB1 at low attomolar levels with the bio-bar-code amplification approach. This is also the first demonstration of a bio-bar code type assay for the detection of AFB1 in Chinese herbs. Keywords: Aflatoxin B1, Bio-bar code assay, Chinese herbs, Magnetic microparticle probes, Nanoparticle probes |
| Databáze: | OpenAIRE |
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