Neutralization of Pseudomonas aeruginosa enzymatic activity by antibodies elicited with proteins of Larrea divaricata Cav

Autor: Roberto Davicino, María Aída Mattar de Anaya, Blas Micalizzi, Renzo F. Martino, Corina Verónica Sasso, Yolanda Casali
Rok vydání: 2012
Předmět:
Cell Extracts
Male
Protein Denaturation
IMMUNE STIMULANTS
medicine.medical_treatment
Toxicology
medicine.disease_cause
PSEUDOMONAS AERUGINOSA
Mice
Immunology and Allergy
Plant Proteins
chemistry.chemical_classification
LARREA DIVARICATA CAV
Plant Stems
Vaccination
Hemolysin
General Medicine
Larrea
Enzymes
Biochemistry
Pseudomonas aeruginosa
Female
AQUEOUS EXTRACT IMMUNOGENIC PROTEINS
Proteases
Immunology
Mice
Inbred Strains
Biology
Cross Reactions
Hemolysis
Microbiology
Bacterial Proteins
medicine
Extracellular
Animals
Humans
Protease Inhibitors
Larrea divaricata
Mercaptoethanol
Pharmacology
Protease
Plant Extracts
Immune Sera
CROSS-REACTION
Antigens
Plant
biology.organism_classification
Antibodies
Neutralizing
Plant Leaves
Enzyme
chemistry
Culture Media
Conditioned
Biocatalysis
Bacteria
Peptide Hydrolases
Zdroj: Immunopharmacology and immunotoxicology. 34(2)
ISSN: 1532-2513
Popis: Larrea divaricata Cav. (Jarilla) is a bush widely used in folk therapy for the treatment of several pathologies. Partially purified proteins of crude extract (JPCE) cross-react with proteins of Gram-negative bacteria, including Pseudomonas aeruginosa, which is an opportunistic pathogen that causes several intrahospitalary infections. This bacterium produces many proteins with enzymatic activity, including hemolysins and proteases that play a major role in acute infection caused by this bacterium. The aim of our work was to investigate if antibodies against with L. divaricata neutralize the hemolytic and proteolytic activity of P. aeruginosa. The hemolytic activity of soluble cellular proteins was inhibited 100% and extracellular proteins (EP) showed an inhibition between 44 and 95% when both bacterial fractions were treated with anti-JPCE serum. Also, in EP the neutralization was directed towards the active site of the hemolysin. When protease activity of extracellular products was tested, bands of 217, 155, 121, 47 and 27kDa were observed in native zymograms. Neutralization between 55 and 70% of the bands of 217, 155 and 121kDa was observed when EP were treated with anti-JPCE serum. In conclusion, our data clearly demonstrate that antibodies elicited with L. divaricata' proteins are able to neutralize the hemolytic and proteolytic activity of P. aeruginosa cellular and extracellular proteins. Our study constitutes the first report that associates the immunogenicity of plant proteins and bacterial proteins with enzymatic activity. These findings could be relevant in the development of alternatives therapies for patients suffering intrahospitalary opportunistic infections with P. aeruginosa. © 2012 Informa Healthcare USA, Inc. Fil: Sasso, Corina Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Mattar Domínguez, María Aída. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Davicino, Roberto Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Martino, Renzo Fabricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Casali, Judith Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Micalizzi, Blas. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Databáze: OpenAIRE
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