Lack of genoprotective effect of phytosterols and conjugated linoleic acids on Caco-2 cells
| Autor: | Nora M. O'Brien, T. Daly, S. Aisling Aherne, Thomas P. O'Connor |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Methylnitronitrosoguanidine
Cell Survival DNA damage Linoleic acid Campesterol Conjugated linoleic acid Enzyme-Linked Immunosorbent Assay Biology Protective Agents Toxicology Linoleic Acid chemistry.chemical_compound Humans L-Lactate Dehydrogenase integumentary system Phytosterol Cell Membrane Phytosterols food and beverages Hydrogen Peroxide General Medicine Sitosterols Comet assay Cholesterol chemistry Biochemistry Cyclooxygenase 2 Caco-2 Cell culture lipids (amino acids peptides and proteins) Comet Assay Caco-2 Cells DNA Damage Mutagens Food Science |
| Zdroj: | Food and Chemical Toxicology. 47:1791-1796 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2009.04.031 |
| Popis: | Much interest has focused on the cholesterol-lowering effects of phytosterols (plant sterols) but limited data suggests they may also possess anti-carcinogenic activity. Conjugated linoleic acids (CLA), sourced from meat and dairy products of ruminant animals, has also received considerable attention as a potential anti-cancer agent. Therefore, the aims of this project were to (i) examine the effects of phytosterols and CLA on the viability and growth of human intestinal Caco-2 cells and (ii) determine their potential genoprotective (comet assay), COX-2 modulatory (ELISA) and apoptotic (Hoechst staining) activities. Caco-2 cells were supplemented with the phytosterols campesterol, beta-sitosterol, or beta-sitostanol, or a CLA mixture, or individual CLA isomers (c10t12-CLA, t9t11-CLA) for 48 h. The three phytosterols, at the highest levels tested, were found to reduce both the viability and growth of Caco-2 cells while CLA exhibited isomer-specific effects. None of the phytosterols protected against DNA damage. At a concentration of 25 microM, both c10t12-CLA and t9t11-CLA enhanced (P |
| Databáze: | OpenAIRE |
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