3D imaging using scanning diffractometry
Autor: | Morteza Jafari Siavashani, Vahid Abbasian, Ehsan Ahadi Akhlaghi, Alireza Moradi, Mohammad A. Charsooghi, Iman Naghedi, Mohammad Taghi Tavassoly |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Diffraction
Materials science Holographic grating Science 02 engineering and technology 01 natural sciences Displacement (vector) Article 010309 optics Optics 0103 physical sciences Sensitivity (control systems) Optical techniques Microscopy Multidisciplinary business.industry Imaging and sensing 021001 nanoscience & nanotechnology Vibration Interferometry Medicine Digital holographic microscopy 0210 nano-technology business Fresnel diffraction |
Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
ISSN: | 2045-2322 |
Popis: | Imaging of cells is a challenging problem as they do not appreciably change the intensity of the illuminating light. Interferometry-based methods to do this task suffer from high sensitivity to environmental vibrations. We introduce scanning diffractometry as a simple non-contact and vibration-immune methodology for quantitative phase imaging. Fresnel diffractometry by a phase step has led to several applications such as high-precision measurements of displacement. Additional scanning may lead to 3D imaging straightforwardly. We apply the technique to acquire 3D images of holographic grating, red blood cell, neuron, and sperm cell. Either visibility of the diffraction fringes or the positions of extrema may be used for phase change detection. The theoretical analysis through the Fresnel diffraction from one-dimensional phase step is presented and the experimental results are validated with digital holographic microscopy. The presented technique can be suggested to serve as a robust device for 3D phase imaging and biomedical measurements. |
Databáze: | OpenAIRE |
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