Enhancement of Immunofluorescent Staining of Erythrocytes by Saponin Hemolysis
| Autor: | Sidney J. Klein, Emmett B. Reilly, Barbara W. Massey |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Erythrocytes
Immunology Saponin Fluorescent Antibody Technique In Vitro Techniques Biology Hemolysis Stain Antigen medicine Humans Immunology and Allergy Antigens chemistry.chemical_classification Quenching (fluorescence) Hematology Saponins medicine.disease Molecular biology Staining carbohydrates (lipids) Biochemistry chemistry Blood Group Antigens biology.protein Indicators and Reagents Hemoglobin Antibody |
| Zdroj: | Transfusion. 5:434-439 |
| ISSN: | 1537-2995 0041-1132 |
| DOI: | 10.1111/j.1537-2995.1965.tb02921.x |
| Popis: | The demonstration of human blood group antigens by the fluorescent antibody technic has been improved by hemolyzing the red cells with saponin. Hemoglobin has a quenching effect on fluorescence. Its removal resulted in dramatic enhancement of the stain. Saponin proved more effective for this purpose than several other common hemolytic agents, all of which caused nonspecific staining. Antigens A, B, M, and N were readily demonstrated when the saponin step was incorporated into the usual two-layer staining procedure. Antigen Rho(D) required the additional enhancement provided by the triple sandwich technic. |
| Databáze: | OpenAIRE |
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