The Synergistic Antitumor Effect of 5-Fluorouracil Combined with Allicin against Lung and Colorectal Carcinoma Cells
| Autor: | Ancuța Jurj, Adrian Bogdan Țigu, Augustin C. Moț, Vlad-Alexandru Toma, Cristian Moldovan, Marcel Pârvu, Ioana Berindan-Neagoe, Eva Fischer-Fodor |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Programmed cell death
Antimetabolites Antineoplastic Colorectal cancer Cell Survival Pharmaceutical Science allicin migration Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry synergistic effect colony Cell Line Tumor Drug Discovery medicine Humans 5-FU Disulfides Physical and Theoretical Chemistry Lung cancer 030304 developmental biology Cell Proliferation 0303 health sciences Allicin Cell growth Organic Chemistry Cell Cycle apoptosis Cell migration Drug Synergism medicine.disease Sulfinic Acids chemistry Chemistry (miscellaneous) Cell culture Apoptosis 030220 oncology & carcinogenesis Cancer research Molecular Medicine Fluorouracil |
| Zdroj: | Molecules Volume 25 Issue 8 Molecules, Vol 25, Iss 1947, p 1947 (2020) |
| ISSN: | 1420-3049 |
| Popis: | 5-fluorouracil (5-FU) is an anticancer drug used to inhibit the proliferation of many different tumor cells. Since severe events are associated with this compound, its combination with different anticancer drugs or adjuvants would allow the use of a significantly lower dose of 5-FU. In this study, we highlighted that the combination of allicin with 5-FU inhibited the cell migration and proliferation of colorectal and lung cancer cells. 5-FU inhibited cell growth with a similar inhibitory concentration for both normal and tumor cells (~200µ M), while allicin showed different inhibitory concentrations. With an IC50 of 8.625 µ M, lung cancer cells were the most sensitive to allicin. Compared to 5-FU and allicin single-agent treatments, the co-treatment showed a reduced viability rate, with p < 0.05. The morphological changes were visible on all three cell lines, indicating that the treatment inhibited the proliferation of both normal and tumor cells. We highlighted different cell death mechanisms&mdash apoptosis for lung cancer and a non-apoptotic cell death for colorectal cancer. The synergistic antitumor effect of 5-FU combined with allicin was visible against lung and colorectal carcinoma cells. Better results were obtained when a lower concentration of 5-FU was combined with allicin than the single-agent treatment at IC50. |
| Databáze: | OpenAIRE |
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