Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells

Autor: Thao Thi Chu, Hue Thi Hong Bui, Anh Viet Bui, Xuan-Hung Nguyen, Huyen Thi Le, Khanh Thi Van Bui, Tu Dac Nguyen, Thu Thi Hoai Do, Cuong Thi Pham, Uyen Thi Trang Than, Phong Van Nguyen, Liem Thanh Nguyen, Nhung Thi My Hoang, Diem Huong Hoang
Rok vydání: 2020
Předmět:
0301 basic medicine
Lung Neoplasms
Apoptosis
Exosomes
Lymphocyte Activation
Monocytes
lcsh:Chemistry
0302 clinical medicine
Cell Movement
CD3+Vγ9 T cells
Tumor Cells
Cultured
Cytotoxic T cell
lcsh:QH301-705.5
Spectroscopy
General Medicine
Fetal Blood
Computer Science Applications
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cord blood
tumor cell lysate-specific-CD8+ T cells
T cell
Peripheral blood mononuclear cell
Exosome
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
medicine
Humans
exosome
dendritic cells
Physical and Theoretical Chemistry
Molecular Biology
Cell Proliferation
Cryopreservation
A549 cell
business.industry
Organic Chemistry
Dendritic cell
Microvesicles
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Cancer research
dendritic cell vaccination
business
T-Lymphocytes
Cytotoxic
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 5
International Journal of Molecular Sciences, Vol 21, Iss 5, p 1834 (2020)
ISSN: 1422-0067
Popis: (1) Background: Dendritic cell (DC) vaccination has shown outstanding achievements in cancer treatment, although it still has some adverse side effects. Vaccination with DC-derived exosomes has been thought to overcome the side effects of the parental DCs. (2) Method: We performed the experiments to check the ability of cryopreserved umbilical cord blood mononuclear cell-derived DCs (cryo CBMDCs) and their exosomes to prime allogeneic T cell proliferation and allogeneic peripheral blood mononuclear cell (alloPBMCs) cytotoxicity against A549 lung cancer cells. (3) Results: We found that both lung tumor cell lysate-pulsed DCs and their exosomes could induce allogeneic T cell proliferation. Moreover, alloPBMCs primed with tumor cell lysate-pulsed DCs and their exosomes have a greater cytotoxic activity against A549 cells compared to unprimed cells and cells primed with unpulsed DCs and their exosomes. (4) Conclusion: Tumor cell lysate-pulsed DCs and their exosomes should be considered to develop into a novel immunotherapeutic strategy&mdash
e.g., vaccines&mdash
for patients with lung cancer. Our results also suggested that cryo umbilical cord blood mononuclear cells source, which is a readily and available source, is effective for generation of allogeneic DCs and their exosomes will be material for vaccinating against cancer.
Databáze: OpenAIRE
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