Quality of molecular detection of vancomycin resistance in enterococci : results of 6 consecutive years of Quality Control for Molecular Diagnostics (QCMD) external quality assessment

Autor: P. Wallace, Calum Scott, Caterina Di Lorenzo, Herman Goossens, Elaine McCulloch, Oliver Donoso Mantke, Veerle Matheeussen, Katherine Loens, Margareta Ieven
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Microbiology (medical)
Quality Control
Veterinary medicine
030106 microbiology
Enterococcus faecium
Drug resistance
Microbial Sensitivity Tests
medicine.disease_cause
Polymerase Chain Reaction
Sensitivity and Specificity
Vancomycin-Resistant Enterococci
03 medical and health sciences
0302 clinical medicine
Bacterial Proteins
External quality assessment
medicine
Proficiency testing
030212 general & internal medicine
Pathology
Molecular
Carbon-Oxygen Ligases
Biology
Gram-Positive Bacterial Infections
Vancomycin resistance
biology
business.industry
Pharmacology. Therapy
Vancomycin Resistance
General Medicine
biochemical phenomena
metabolism
and nutrition
Molecular diagnostics
biology.organism_classification
bacterial infections and mycoses
Anti-Bacterial Agents
Infectious Diseases
Staphylococcus aureus
Vancomycin
Human medicine
business
medicine.drug
Zdroj: European journal of clinical microbiology and infectious diseases
European Journal of Clinical Microbiology & Infectious Diseases
ISSN: 0934-9723
Popis: The quality of PCR to detect vancomycin-resistant enterococci (VRE) was evaluated by analysing their performance in six consecutive external quality assessment (EQA) schemes, organized annually since 2013 by Quality Control for Molecular Diagnostics. VRE EQA panels consisted of 12–14 heat-inactivated samples. Sensitivity was tested with vanA-positive Enterococcus faecium (E. faecium), vanB-positive E. faecium, E. faecalis or E. gallinarum or vanC-positive E. gallinarum in different concentrations. Vancomycin-susceptible enterococci, Staphylococcus aureus or sample matrix was used to study the specificity. Participants were asked to report the VRE resistance status of each sample. The detection rate of vanA-positive samples was already 95% in the 2013 EQA panel (range 94–97%) and remained stable over the years. The 2013 detection rate of vanB-positive samples was 82% but increased significantly by more than 10% in subsequent years (96% in 2014, 95% in 2015, 92% in 2016 and 93% in 2017/2018, p
Databáze: OpenAIRE
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