A non-coding small RNA MicC contributes to virulence in outer membrane proteins in Salmonella Enteritidis
| Autor: | Guoqiang Zhu, Weiwei Cui, Jinqiu Wang, Xianchen Meng, Xia Meng, Jianye Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Salmonella typhimurium
Salmonella RNA Untranslated General Chemical Engineering Salmonella enteritidis Mutant Virulence Biology Real-Time Polymerase Chain Reaction medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology Mice Bacterial Proteins Gene expression medicine Animals RNA Messenger Escherichia coli Gene Recombination Genetic Mice Inbred BALB C Salmonella Infections Animal General Immunology and Microbiology General Neuroscience Membrane Proteins Gene Expression Regulation Bacterial RNA Bacterial Mutation Bacterial outer membrane Chickens Plasmids |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/61808 |
| Popis: | A non-coding small RNA (sRNA) is a new factor to regulate gene expression at the post-transcriptional level. A kind of sRNA MicC, known in Escherichia coli and Salmonella Typhimurium, could repress the expression of outer membrane proteins. To further investigate the regulation function of micC in Salmonella Enteritidis, we cloned the micC gene in the Salmonella Enteritidis strain 50336, and then constructed the mutant 50336ΔmicC by the λ Red-based recombination system and the complemented mutant 50336ΔmicC/pmicC carrying recombinant plasmid pBR322 expressing micC. qRT-PCR results demonstrated that transcription of ompD in 50336ΔmicC was 1.3-fold higher than that in the wild type strain, while the transcription of ompA and ompC in 50336ΔmicC were 2.2-fold and 3-fold higher than those in the wild type strain. These indicated that micC represses the expression of ompA and ompC. In the following study, the pathogenicity of 50336ΔmicC was detected by both infecting 6-week-old Balb/c mice and 1-day-old chickens. The result showed that the LD50 of the wild type strain 50336, the mutants 50336ΔmicC and 50336ΔmicC/pmicC for 6-week-old Balb/c mice were 12.59 CFU, 5.01 CFU, and 19.95 CFU, respectively. The LD50 of the strains for 1-day-old chickens were 1.13 x 109 CFU, 1.55 x 108 CFU, and 2.54 x 108 CFU, respectively. It indicated that deletion of micC enhanced virulence of S. Enteritidis in mice and chickens by regulating expression of outer membrane proteins. |
| Databáze: | OpenAIRE |
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