Independently Controlling Protein Dot Size and Spacing in Particle Lithography
| Autor: | David W. Schmidtke, Joel C. Keay, Ernest S. Sanchez, Zachary R. Taylor, Matthew B. Johnson |
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| Rok vydání: | 2012 |
| Předmět: |
Hot Temperature
Materials science Neutrophils Nanotechnology chemistry.chemical_compound Cell Adhesion Electrochemistry Humans General Materials Science Colloids Lithography Spectroscopy Cell Size chemistry.chemical_classification Membrane Glycoproteins business.industry Biomolecule Proteins Surfaces and Interfaces Polymer Condensed Matter Physics Immobilized Proteins src-Family Kinases chemistry Printing Optoelectronics Particle Nanometre SPHERES Differential heating business Ethylene glycol |
| Zdroj: | Langmuir. 28:9656-9663 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | Particle lithography is a relatively simple, inexpensive technique used to pattern inorganics, metals, polymers, and biological molecules on the micro- and nanometer scales. Previously, we used particle lithography to create hexagonal patterns of protein dots in a protein resistant background of methoxy-poly(ethylene glycol)-silane (mPEG-sil). In this work, we describe a simple heating procedure to overcome a potential limitation of particle lithography: the simultaneous change in feature size and center-to-center spacing as the diameter of the spheres used in the lithographic mask is changed. Uniform heating was used to make single-diameter protein patterns with dot sizes of approximately 2-4 or 2-8 μm, depending on the diameter of the spheres used in the lithographic mask, while differential heating was used to make a continuous gradient of dot sizes of approximately 1-9 μm on a single surface. We demonstrate the applicability of these substrates by observing the differences in neutrophil spreading on patterned and unpatterned protein coated surfaces. |
| Databáze: | OpenAIRE |
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