Simple and fast multiplex PCR method for detection of species origin in meat products
| Autor: | Parvaneh Farzaneh, Seyed Abolhassan Shahzadeh Fazeli, Zahra Elyasi Gorji, Faezeh Vakhshiteh, Mehrnaz Izadpanah, Nazanin Mohebali |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pcr cloning 04 agricultural and veterinary sciences Computational biology Biology 040401 food science Animal origin law.invention 03 medical and health sciences 030104 developmental biology 0404 agricultural biotechnology law Specific primers Multiplex polymerase chain reaction Species identification Identification (biology) Original Article Animal species Polymerase chain reaction Food Science |
| Popis: | Identification of animal species is one of the major concerns in food regulatory control and quality assurance system. Different approaches have been used for species identification in animal origin of feedstuff. This study aimed to develop a multiplex PCR approach to detect the origin of meat and meat products. Specific primers were designed based on the conserved region of mitochondrial Cytochrome C Oxidase subunit I (COX1) gene. This method could successfully distinguish the origin of the pig, camel, sheep, donkey, goat, cow, and chicken in one single reaction. Since PCR products derived from each species represent unique molecular weight, the amplified products could be identified by electrophoresis and analyzed based on their size. Due to the synchronized amplification of segments within a single PCR reaction, multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for identification of meat products in food industries. Nowadays, this technique has been considered as a practical method to identify the species origin, which could further applied for animal feedstuffs identification. |
| Databáze: | OpenAIRE |
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