Identification of passion fruit (Passiflora edulis) chromosomes using BAC-FISH

Autor: Y. Dias, Carla de Freitas Munhoz, Zirlane Portugal da Costa, Maria Lucia Carneiro Vieira, Mariela Sader, Andrea Pedrosa-Harand, Helen A. Penha, Hélène Bergès
Přispěvatelé: Universidade Federal de Pernambuco [Recife] (UFPE), Universidade de São Paulo (USP), Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), National Council for Scientific and Technological Development (CNPq) [310804/2017-5], Universidade Federal de Pernambuco (UFPE), Universidade Estadual Paulista (Unesp), French Plant Genomic Resources Center (CNRGV)/ INRA
Rok vydání: 2019
Předmět:
Genetic Markers
0106 biological sciences
Retroelements
Karyotype
Retrotransposon
bacterial artificial chromosome (BAC)
Biology
01 natural sciences
Genome
Chromosomes
Plant
Passiflora
fluorescent in situ hybridization (FISH)
03 medical and health sciences
Genetics
cytogenetic map
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Genomic library
Gene
In Situ Hybridization
Fluorescence
Repetitive Sequences
Nucleic Acid
030304 developmental biology
2. Zero hunger
Bacterial artificial chromosome (BAC)
repetitive DNA sequences
0303 health sciences
Bacterial artificial chromosome
retrotransposon
Chromosome Mapping
food and beverages
Chromosome
biology.organism_classification
Cytogenetic map
karyotype
Fluorescent in situ hybridization (FISH)
Repetitive DNA sequences
SEQUÊNCIA DO DNA
010606 plant biology & botany
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Chromosome Research
Chromosome Research, Springer Verlag, 2019, 27 (4), pp.299-311. ⟨10.1007/s10577-019-09614-0⟩
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1573-6849
0967-3849
DOI: 10.1007/s10577-019-09614-0
Popis: Made available in DSpace on 2019-10-06T15:50:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Passiflora edulis, the yellow passion fruit, is the main crop from the Passiflora genus, which comprises 525 species with its diversity center in South America. Genetic maps and a BAC (bacterial artificial chromosome) genomic library are available, but the nine chromosome pairs of similar size and morphology (2n = 18) hamper chromosome identification, leading to different proposed karyotypes. Thus, the aim of this study was to establish chromosome-specific markers for the yellow passion fruit using single-copy and repetitive sequences as probes in fluorescent in situ hybridizations (FISH) to allow chromosome identification and future integration with whole genome data. Thirty-six BAC clones harboring genes and three retrotransposons (Ty1-copy, Ty3-gypsy, and LINE) were selected. Twelve BACs exhibited a dispersed pattern similar to that revealed by retroelements, and one exhibited subtelomeric distribution. Twelve clones showed unique signals in terminal or subterminal regions of the chromosomes, allowing their genes to be anchored to six chromosome pairs that can be identified with single-copy markers. The markers developed herein will provide an important tool for genomic and evolutionary studies in the Passiflora genus. Department of Botany Federal University of Pernambuco Department of Genetics “Luiz de Queiroz” College of Agriculture University of São Paulo Department of Technology Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP) French Plant Genomic Resources Center (CNRGV)/ INRA Laboratório de Citogenética e Evolução Vegetal Departamento de Botânica Centro de Biociências Universidade Federal de Pernambuco, R. Prof. Moraes Rego, s/n, CDU Department of Technology Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
Databáze: OpenAIRE
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