Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord
| Autor: | Seok-Yong Choi, Min Jung Kim, Jin Young Bae, In Young Jeong, Seung Woo Park, Jun Zhang, Dong-Young Kim, Yong Chul Bae, So Hyun Lee, Hyunju Ro, Jin Soo Maeng, Hae Chul Park, Isaac Choi, Hyuno Kang, Eun-Young Choi, Myeong Kyu Kim, Hyung-Seok Kim, Gopalakrishnan Chandrasekaran, Taewon Lee, Wenting Li, Ting Liang |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Ependymal Cell Science General Physics and Astronomy Connexin Biology General Biochemistry Genetics and Molecular Biology Article Connexins 03 medical and health sciences Gene Knockout Techniques Mice 0302 clinical medicine Ependyma medicine Basal body Animals Humans Cilia lcsh:Science Zebrafish Wnt Signaling Pathway Mice Knockout Multidisciplinary Ciliogenesis Wnt signaling pathway Gap Junctions Gene Expression Regulation Developmental Cell Differentiation General Chemistry Zebrafish Proteins medicine.disease biology.organism_classification Cell biology Neuroepithelial cell Ciliopathy 030104 developmental biology Spinal Cord Differentiation Connexin 43 Motile cilium lcsh:Q 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020) |
| ISSN: | 2041-1723 |
| Popis: | Ependymal cells (ECs) are multiciliated neuroepithelial cells that line the ventricles of the brain and the central canal of the spinal cord (SC). How ependymal motile cilia are maintained remains largely unexplored. Here we show that zebrafish embryos deficient in Wnt signaling have defective motile cilia, yet harbor intact basal bodies. With respect to maintenance of ependymal motile cilia, plcδ3a is a target gene of Wnt signaling. Lack of Connexin43 (Cx43), especially its channel function, decreases motile cilia and intercellular Ca2+ wave (ICW) propagation. Genetic ablation of cx43 in zebrafish and mice diminished motile cilia. Finally, Cx43 is also expressed in ECs of the human SC. Taken together, our findings indicate that gap junction mediated ICWs play an important role in the maintenance of ependymal motile cilia, and suggest that the enhancement of functional gap junctions by pharmacological or genetic manipulations may be adopted to ameliorate motile ciliopathy. Ependymal cells are supporting cells in the central nervous system. Here the authors elucidate a signalling axis in zebrafish spinal cord ependymal cells that is important for motile cilia assembly and maintenance, demonstrating that it depends on intercellular propagation of calcium ions via connexin 43. |
| Databáze: | OpenAIRE |
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