Co-expression of CYP27B1 enzyme with the 1.5kb CYP27B1 promoter-luciferase transgene in the mouse
| Autor: | Brian K. May, Reza Zarrinkalam, Howard A. Morris, Paul H. Anderson, Jim Manavis, Ghafar T. Sarvestani, Ivanka Hendrix, Rebecca Sawyer |
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| Přispěvatelé: | Anderson, Paul Hamill, Hendrix, Ivanka, Sawyer, R.K, Zarrinkalam, Reza, Manavis, Jim, Sarvestani, Ghafar T, May, Brian, Morris, Howard Arthur |
| Rok vydání: | 2007 |
| Předmět: |
Genetically modified mouse
Male 25-hydroxyvitamin D-1alpha hydroxylase 5' Flanking Region Transgene Recombinant Fusion Proteins 25-Hydroxyvitamin D3 1-alpha-hydroxylase 5' flanking region Mice Transgenic Biology Medical Biochemistry and Metabolomics Biochemistry Gene Expression Regulation Enzymologic Mice Endocrinology medicine polycyclic compounds Animals Humans Luciferase Tissue Distribution Transgenes Promoter Regions Genetic Molecular Biology Gene Regulation of gene expression 25-Hydroxyvitamin D3 1-alpha-Hydroxylase luciferase Molecular biology Epithelium medicine.anatomical_structure embryonic structures lipids (amino acids peptides and proteins) |
| Zdroj: | Molecular and cellular endocrinology. 285(1-2) |
| ISSN: | 0303-7207 |
| Popis: | The renal enzyme 25-hydroxyvitamin D 1alpha-hydroxylase (CYP27B1), responsible for the synthesis of circulating. 1,25-dihydroxyvitamin D (1,25D), is also expressed in a number of non-renal tissues. The regulation of CYP27B1 expression by the short flanking promoter outside the kidney is, however, largely unknown. We have used a transgenic mice expressing the 1.5kb promoter of the human CYP27B1 gene fused to the firefly luciferase gene in order to investigate tissue-specific CYP27B1 expression. These transgenic animals demonstrated co-localised luciferase and endogenous CYP27B1 expression in kidney proximal convoluted tubular cells. Strong co-expression of luciferase and CYP27B1 also occurred in neurons and Purkinje cells of the cerebellum and in Leydig and Sertoli cells of the testes. Other tissues to exhibit CYP27B1-promoter directed luciferase activity included lung, prostate, trabecular bone and jejunum as well as the choroid epithelium. The tissue specific changes in luciferase activity were age-related. These findings demonstrate that the proximal 1.5kb 5' flanking region of the CYP27B1 gene directs the expression of CYP27B1 in a number of known and novel tissues in a specific manner. |
| Databáze: | OpenAIRE |
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