Single cell-derived spheroids capture the self-renewing subpopulations of metastatic ovarian cancer
| Autor: | Tania Velletri, Marco De Simone, Luca Marelli, Raffaele Luongo, Stefano Piccolo, Nicoletta Colombo, Michela Lupia, Carlo Emanuele Villa, Ugo Cavallaro, Pietro Lo Riso, Raoul J. P. Bonnal, Alejandro López-Tobón, Massimiliano Pagani, Domenica Cilli, Bianca Barzaghi, Giuseppe Testa, Saverio Minucci |
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| Přispěvatelé: | Velletri, T, Villa, C, Cilli, D, Barzaghi, B, Lo Riso, P, Lupia, M, Luongo, R, Lopez-Tobon, A, De Simone, M, Bonnal, R, Marelli, L, Piccolo, S, Colombo, N, Pagani, M, Cavallaro, U, Minucci, S, Testa, G |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Biochemistry & Molecular Biology
Cell Biology Metastasis Unmet needs Cell Line Molecular level Cell Line Tumor Spheroids Cellular ORGANOIDS medicine Serous ovarian cancer Female Humans Precision Medicine Ascites Ovarian Neoplasms Molecular Biology Science & Technology Tumor Spheroid EXPANSION Cell Biology FIT medicine.disease EPITHELIAL-MESENCHYMAL TRANSITION medicine.anatomical_structure High Grade Serous Ovarian cancer Cancer research Cellular Spheroids Ovarian cancer Life Sciences & Biomedicine STEM-CELLS BEHAVIOR Metastatic ovarian cancer |
| Popis: | High Grade Serous Ovarian cancer (HGSOC) is a major unmet need in oncology, due to its precocious dissemination and the lack of meaningful human models for the investigation of disease pathogenesis in a patient-specific manner. To overcome this roadblock, we present a new method to isolate and grow single cells directly from patients' metastatic ascites, establishing the conditions for propagating them as 3D cultures that we refer to as single cell-derived metastatic ovarian cancer spheroids (sMOCS). By single cell RNA sequencing (scRNAseq) we define the cellular composition of metastatic ascites and trace its propagation in 2D and 3D culture paradigms, finding that sMOCS retain and amplify key subpopulations from the original patients' samples and recapitulate features of the original metastasis that do not emerge from classical 2D culture, including retention of individual patients' specificities. By enabling the enrichment of uniquely informative cell subpopulations from HGSOC metastasis and the clonal interrogation of their diversity at the functional and molecular level, this method provides a powerful instrument for precision oncology in ovarian cancer. ispartof: CELL DEATH AND DIFFERENTIATION vol:29 issue:3 pages:614-626 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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