Dairy Product Consumption Interacts with Glucokinase (GCK) Gene Polymorphisms Associated with Insulin Resistance
| Autor: | Marine S. Da Silva, Marie-Claude Vohl, Olivier Barbier, Iwona Rudkowska, Dominic Chartrand |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Medicine (miscellaneous) lcsh:Medicine 030209 endocrinology & metabolism Carbohydrate metabolism Biology Article Whey protein isolate 03 medical and health sciences 0302 clinical medicine Insulin resistance nutrigenomics Valine medicine insulin sensitivity glucose 030109 nutrition & dietetics Glucokinase lcsh:R medicine.disease dairy type 2 diabetes Fatty acid synthase Insulin receptor Biochemistry biology.protein Leucine |
| Zdroj: | Journal of Personalized Medicine, Vol 7, Iss 3, p 8 (2017) Journal of Personalized Medicine Journal of Personalized Medicine; Volume 7; Issue 3; Pages: 8 |
| ISSN: | 2075-4426 |
| Popis: | Dairy product intake and a person’s genetic background have been reported to be associated with the risk of type 2 diabetes (T2D). The objective of this study was to examine the interaction between dairy products and genes related to T2D on glucose-insulin homeostasis parameters. A validated food frequency questionnaire, fasting blood samples, and glucokinase (GCK) genotypes were analyzed in 210 healthy participants. An interaction between rs1799884 in GCK and dairy intake on the homeostasis model assessment of insulin resistance was identified. Secondly, human hepatocellular carcinoma cells (HepG2) were grown in a high-glucose medium and incubated with either 1-dairy proteins: whey, caseins, and a mixture of whey and casein; and 2-four amino acids (AA) or mixtures of AA. The expression of GCK-related genes insulin receptor substrate-1 (IRS-1) and fatty acid synthase (FASN) was increased with whey protein isolate or hydrolysate. Individually, leucine increased IRS-1 expression, whereas isoleucine and valine decreased FASN expression. A branched-chain AA mixture decreased IRS-1 and FASN expression. In conclusion, carriers of the A allele for rs1799884 in the GCK gene may benefit from a higher intake of dairy products to maintain optimal insulin sensitivity. Moreover, the results show that whey proteins affect the expression of genes related to glucose metabolism. |
| Databáze: | OpenAIRE |
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