Dietary Strontium Increases Bone Mineral Density in Intact Zebrafish (Danio rerio): A Potential Model System for Bone Research
| Autor: | Stephen A. Watts, Steve Padgett-Vasquez, Louis R. D'Abramo, Heath W. Garris, Tim R. Nagy, Anthony J. Siccardi |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
X-ray microtomography animal structures Bone density Danio chemistry.chemical_element Mineralization (biology) Bone and Bones Bone Density Internal medicine medicine Animals Zebrafish Bone mineral Strontium biology Original Articles X-Ray Microtomography biology.organism_classification Spinal column Animal Feed Endocrinology chemistry Animal Science and Zoology Developmental Biology |
| Popis: | Zebrafish (Danio rerio) skeletal bone possesses properties similar to human bone, which suggests that they may be used as a model to study mineralization characteristics of the human Haversian system, as well as human bone diseases. One prerequisite for the use of zebrafish as an alternative osteoporotic bone model is to determine whether their bone displays functional plasticity similar to that observed in other bone models. Strontium citrate was supplemented into a laboratory-prepared diet (45% crude protein) to produce dietary strontium levels of 0%, 0.63%, 1.26%, 1.89%, and 2.43% and fed ad libitum twice daily for 12 weeks to 28-day-old intact zebrafish. Length was determined at 4-week intervals, and both weight and length were recorded at 12 weeks. At 12 weeks, seven zebrafish from each dietary level were analyzed for total bone mineral density by microcomputed tomography. Dietary strontium citrate supplementation significantly (p |
| Databáze: | OpenAIRE |
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