Multifamily Determination of Phytohormones and Acidic Herbicides in Fruits and Vegetables by Liquid Chromatography–Tandem Mass Spectrometry under Accredited Conditions
| Autor: | María Elena Hernández Torres, Rosario Santiago Valverde, Ángel Grande Martínez, Antonia Garrido Frenich, Francisco J. Arrebola Liébanas, Juan Ramírez Casinello |
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| Rok vydání: | 2020 |
| Předmět: |
Dichlorprop
Analyte Health (social science) fruits and vegetables UHPLC–MS/MS Plant Science Orange (colour) multifamily method lcsh:Chemical technology 010402 general chemistry Tandem mass spectrometry Quechers 01 natural sciences Health Professions (miscellaneous) Microbiology MCPA Article chemistry.chemical_compound Acetic acid Liquid chromatography–mass spectrometry lcsh:TP1-1185 Chromatography 010401 analytical chemistry 0104 chemical sciences phytohormones chemistry acidic herbicides Food Science |
| Zdroj: | riUAL. Repositorio Institucional de la Universidad de Almería Universidad de Almería Foods Volume 9 Issue 7 Foods, Vol 9, Iss 906, p 906 (2020) |
| Popis: | A 7-min multifamily residue method for the simultaneous quantification and confirmation of 8 phytohormones and 27 acidic herbicides in fruit and vegetables using ultra high-performance liquid chromatography (UHPLC) coupled to tandem mass spectrometry (MS/MS) was developed, validated according to SANTE 12682/2019, and accredited according to UNE-EN-ISO/IEC 17025:2017. Due to the special characteristics of these kinds of compounds, a previous step of alkaline hydrolysis was carried out for breaking conjugates that were potentially formed due to the interactions of the analytes with other components present in the matrix. Sample treatment was based on QuEChERS extraction and optimum detection conditions were individually optimized for each analyte. Cucumber (for high water content commodities) and orange (for high acid and high water content samples) were selected as representative matrices. Matrix-matched calibration was used, and all the validation criteria established in the SANTE guidelines were satisfied. Uncertainty estimation for each target compound was included in the validation process. The proposed method was applied to the analysis of more than 450 samples of cucumber, orange, tomato, watermelon, and zucchini during one year. Several compounds, such as 2,4-dichlorophenoxyacetic acid (2,4-D), 4-(3-indolyl)butyric acid (IBA), dichlorprop (2,4-DP), 2-methyl-4-chlorophenoxy acetic acid (MCPA), and triclopyr were found, but always at concentrations lower than the maximum residue level (MRL) regulated by the EU. |
| Databáze: | OpenAIRE |
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