CSF1R Protein Expression in Reactive Lymphoid Tissues and Lymphoma: Its Relevance in Classical Hodgkin Lymphoma
| Autor: | Juan F. García, Jose Francisco Tomas, Ana M. Martín-Moreno, Jorge L. Martínez-Torrecuadrada, Carmen Rubio, Lorena Maestre, Karen Pulford, Giovanna Roncador, Miguel A. Piris, Scherezade Jiménez, Ana Isabel Reyes-García, Mónica Estévez, Elena Mata |
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| Přispěvatelé: | Asociación Española Contra el Cáncer, Unión Europea, Ministerio de Ciencia e Innovación (España), Asociacion Espanola Contra el Cancer (AECC), European Union (EU), Ministerio de Ciencia, Innovación (España) |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty PROGNOSIS Lymphoma Lymphoid Tissue FACTOR-I lcsh:Medicine Gene Expression PROGRESSION MICROENVIRONMENT Receptor Macrophage Colony-Stimulating Factor macromolecular substances Biology medicine.disease_cause Mice BCL9 immune system diseases hemic and lymphatic diseases Cell Line Tumor polycyclic compounds medicine Animals Humans Immunoprecipitation lcsh:Science REED-STERNBERG CELLS Anaplastic large-cell lymphoma Multidisciplinary CD68 lcsh:R food and beverages COLONY-STIMULATING FACTOR medicine.disease Epstein–Barr virus Hodgkin Disease Immunohistochemistry BCL10 TUMOR-ASSOCIATED MACROPHAGES Lymphatic system Cancer research SURVIVAL lcsh:Q INHIBITORS CD163 FOLLICULAR LYMPHOMA Research Article Signal Transduction |
| Zdroj: | Repisalud Instituto de Salud Carlos III (ISCIII) PLoS ONE PLoS ONE, Vol 10, Iss 6, p e0125203 (2015) |
| Popis: | Tumour-associated macrophages (TAMs) have been associated with survival in classic Hodgkin lymphoma (cHL) and other lymphoma types. The maturation and differentiation of tissue macrophages depends upon interactions between colony-stimulating factor 1 receptor (CSF1R) and its ligands. There remains, however, a lack of consistent information on CSF1R expression in TAMs. A new monoclonal antibody, FER216, was generated to investigate CSF1R protein distribution in formalin fixed tissue samples from 24 reactive lymphoid tissues and 187 different lymphoma types. We also analysed the distribution of CSF1R+, CD68+ and CD163+ macrophages by double immunostaining, and studied the relationship between CSF1R expression and survival in an independent series of 249 cHL patients. CSF1R+ TAMs were less frequent in B-cell lymphocytic leukaemia and lymphoblastic B-cell lymphoma than in diffuse large B-cell lymphoma, peripheral T-cell lymphoma, angioimmunoblastic T-cell lymphoma and cHL. HRS cells in cHL and, with the exception of three cases of anaplastic large cell lymphoma, the neoplastic cells in NHLs, lacked detectable CSF1R protein. A CSF1R+ enriched microenvironment in cHL was associated with shorter survival in an independent series of 249 cHL patients. CSF1R pathway activation was evident in the cHL and inactivation of this pathway could be a potential therapeutic target in cHL cases. This work was supported by grants from the Fondo de Investigaciones Sanitarias - Ministerio de Ciencia e Innovacion (PI12/1832), and Spanish Cancer Research Networks-RTICC (RD12/0036/0060), cofunded by the Fondo Europeo de Desarrollo Regional (FEDER), and the Asociacion Espanola Contra el Cancer (AECC Scientific Foundation). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Sí |
| Databáze: | OpenAIRE |
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