Ultra-slim plastic endomicroscope objective for non-linear microscopy
| Autor: | Matthew Kyrish, Brenda Baggett, Urs Utzinger, Michael R. Descour, Tomasz S. Tkaczyk |
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| Rok vydání: | 2011 |
| Předmět: |
Point spread function
Diagnostic Imaging Optics and Photonics ocis:(180.4315) Nonlinear microscopy Materials science Aperture 02 engineering and technology Molding (process) 01 natural sciences Signal 010309 optics Optics 0103 physical sciences Microscopy ocis:(170.2150) Endoscopic imaging Lenses Birefringence business.industry Endoscopy Signal Processing Computer-Assisted 021001 nanoscience & nanotechnology Fluorescence Atomic and Molecular Physics and Optics Convallaria Autofluorescence Microscopy Fluorescence Research-Article ocis:(170.3880) Medical and biological imaging 0210 nano-technology business ocis:(350.3950) Micro-optics Rhizome |
| Zdroj: | Optics Express |
| ISSN: | 1094-4087 |
| Popis: | Non-linear microscopy has the potential to provide clinically useful information on the structure of biological tissue in vivo via an endomicroscope. The ability to use plastic as the optical material in a multiphoton objective was evaluated based on several criteria including autofluorescence, injection molding induced birefringence, and pulse broadening due to group velocity dispersion. An all-plastic, refractive ultra-slim endoscope objective was built with design specifications of NA = 0.4, FOV = 250 μm, 1.27 mm outer diameter, and 0.8 mm clear aperture. Initial images of second-harmonic generation signal (illumination at 780 nm) in collagen fibers and two-photon excited fluorescence (illumination at 920 nm) of Convallaria rhizome are reported. |
| Databáze: | OpenAIRE |
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