KChIP3 N-Terminal 31-50 Fragment Mediates Its Association with TRPV1 and Alleviates Inflammatory Hyperalgesia in Rats
| Autor: | Xiao-Hong Sun, Lu Li, Yun Wang, Jin-Yu Yang, Na-Xi Tian, Ying Zhang, Yu Xu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Freund's Adjuvant TRPV1 TRPV Cation Channels Inflammation Epigenetic Repression 03 medical and health sciences Transient receptor potential channel chemistry.chemical_compound Transactivation Gene Knockout Techniques 0302 clinical medicine Dorsal root ganglion Ganglia Spinal medicine Animals Calcium Signaling Gait Injections Spinal Research Articles Pain Measurement Chemistry General Neuroscience Kv Channel-Interacting Proteins Potassium channel Peptide Fragments Cell biology Rats 030104 developmental biology medicine.anatomical_structure Hyperalgesia Calsenilin medicine.symptom 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(7) |
| ISSN: | 1529-2401 |
| Popis: | Potassium voltage-gated channel interacting protein 3 (KChIP3), also termed downstream regulatory element antagonist modulator (DREAM) and calsenilin, is a multifunctional protein belonging to the neuronal calcium sensor (NCS) family. Recent studies revealed the expression of KChIP3 in dorsal root ganglion (DRG) neurons, suggesting the potential role of KChIP3 in peripheral sensory processing. Herein, we show that KChIP3 colocalizes with transient receptor potential ion channel V1 (TRPV1), a critical molecule involved in peripheral sensitization during inflammatory pain. Furthermore, the N-terminal 31-50 fragment of KChIP3 is capable of binding both the intracellular N and C termini of TRPV1, which substantially decreases the surface localization of TRPV1 and the subsequent Ca2+influx through the channel. Importantly, intrathecal administration of the transmembrane peptide transactivator of transcription (TAT)-31-50 remarkably reduces Ca2+influx via TRPV1 in DRG neurons and alleviates thermal hyperalgesia and gait alterations in a complete Freund's adjuvant-induced inflammatory pain model in male rats. Moreover, intraplantar injection of TAT-31-50 attenuated the capsaicin-evoked spontaneous pain behavior and thermal hyperalgesia, which further strengthened the regulatory role of TAT-31-50 on TRPV1 channel. In addition, TAT-31-50 could also alleviate inflammatory thermal hyperalgesia inkcnip3−/−rats generated in our study, suggesting that the analgesic effect mediated by TAT-31-50 is independent of endogenous KChIP3. Our study reveals a novel peripheral mechanism for the analgesic function of KChIP3 and provides a potential analgesic agent, TAT-31-50, for the treatment of inflammatory pain.SIGNIFICANCE STATEMENTInflammatory pain arising from inflamed or injured tissues significantly compromises the quality of life in patients. This study aims to elucidate the role of peripheral potassium channel interacting protein 3 (KChIP3) in inflammatory pain. Direct interaction of the KChIP3 N-terminal 31-50 fragment with transient receptor potential ion channel V1 (TRPV1) was demonstrated. The KChIP3–TRPV1 interaction reduces the surface localization of TRPV1 and thus alleviates heat hyperalgesia and gait alterations induced by peripheral inflammation. Furthermore, the transmembrane transactivator of transcription (TAT)-31-50 peptide showed analgesic effects on inflammatory hyperalgesia independently of endogenous KChIP3. This work reveals a novel mechanism of peripheral KChIP3 in inflammatory hyperalgesia that is distinct from its classical role as a transcriptional repressor in pain modulation. |
| Databáze: | OpenAIRE |
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