Measurement of the α-mannosidase activities in human plasma by a differential assay
| Autor: | A.D. Patrick, Bryan Winchester, Shirish Hirani |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Adult
Male Mannosidase medicine.medical_specialty Hot Temperature Adolescent Genotype Clinical Biochemistry Positive correlation Biochemistry Mannosidosis Sex Factors Internal medicine Mannosidases Methods medicine Humans Child Chemistry Biochemistry (medical) Age Factors Infant Cobalt General Medicine Hydrogen-Ion Concentration Kinetics Zinc Endocrinology Human plasma Child Preschool Immunology Female Mannose Carbohydrate Metabolism Inborn Errors |
| Zdroj: | Clinica Chimica Acta. 81:135-144 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(77)90005-5 |
| Popis: | A differential assay based on their difference in thermal stability has been used to measure the acidic and true intermediate alpha-mannosidases in the plasma of cntrols and individuals homozygous or heterozygous for mannosidosis. The intermediate activity was found to be independent of age, sex or mannosidosis genotype. The acidic alpha-mannosidase did not vary significantly with age or between sexes for groups of the same age. The concentrations of acidic and intermediate alpha-mannosidase showed a positive correlation for adults but not for children. The ratio of acidic to true intermediate alpha-mannosidase might therefore be a useful secondary test for the detection of adult heterozygotes for mannosidosis. |
| Databáze: | OpenAIRE |
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