Myocyte enhancer factor 2C upregulates MASH-1 expression and induces neurogenesis in P19 cells
| Autor: | Sharon Wilton, Ilona S. Skerjanc |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Mef2
Neurogenesis Biophysics Fluorescent Antibody Technique Neurogenins Biochemistry Mice Sp3 transcription factor Structural Biology Basic Helix-Loop-Helix Transcription Factors Tumor Cells Cultured Genetics Animals MEF2C Molecular Biology Transcription factor Cell Aggregation biology MEF2 Transcription Factors Gene Expression Regulation Developmental food and beverages Cell Differentiation Cell Biology Molecular biology DNA-Binding Proteins P19 cell Myogenic Regulatory Factors Embryonal carcinoma embryonic structures biology.protein MEF2 NeuN Transcription Transcription Factors Mash-1 |
| Zdroj: | FEBS Letters. 472:53-56 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(00)01438-1 |
| Popis: | MEF2C is a transcription factor expressed in neural lineages. After transient transfection, the MEF2 family of factors can act synergistically with the neural-specific transcription factor, MASH-1, and activate exogenous neural-specific promoters. To determine whether MEF2C is capable of modulating endogenous gene expression, P19 cell lines were analyzed that overexpressed MEF2C, termed P19[MEF2C] cells. Here we show that P19[MEF2C] cells differentiate into neurons when aggregated with ME2SO. MEF2C-induced neurons expressed neurofilament protein, the nuclear antigen NeuN, as well as MASH-1. Our results indicate that MEF2C can directly or indirectly activate the expression of MASH-1, leading to neurogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text