Human thymic dendritic cell-thymocyte association: ultrastructural cell phenotype analysis
| Autor: | Nicole Paquette, M Pelletier, Hughes Barthélémy, Claude Chartrand, Serge Montplaisir, Diane Landry, Marielle Lafontaine |
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| Rok vydání: | 1989 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Pathology medicine.medical_specialty CD3 Complex T-Lymphocytes CD3 Immunology Receptors Antigen T-Cell CD1 Cell Communication Thymus Gland Biology Antigens CD1 Immunolabeling Cell–cell interaction medicine Humans Immunology and Allergy Dendritic Cells HLA-DR Antigens Dendritic cell Antigens Differentiation Cell biology Microscopy Electron Thymocyte Phenotype Cell culture Antigens Surface biology.protein CD8 |
| Zdroj: | European Journal of Immunology. 19:1855-1860 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830191015 |
| Popis: | In rodent thymus, associations between dendritic cells (DC) and thymocytes have been suggested to be implicated in differentiation and/or maturation processes. In this study, we report intimate associations formed between human thymic DC and thymocytes in culture and we analyze their ultrastructural cell phenotype. Observations by phase contrast microscopy showed that DC present long and thin dendrites and bind many thymocytes. Transmission (TEM) and scanning electron microscopy (SEM) revealed that both cellular populations were in close connection and tight membrane contact could be observed. The phenotype of DC and attached thymocytes was characterized with a series of monoclonal antibodies by protein A-gold TEM and SEM immunolabelings. Quantitative evaluation of immunolabeling (number of gold granules/microns of cellular membrane) suggests the presence of two subpopulations of CD1+ thymic DC (strong and weak), whereas this discrepancy is not observed in DR+ and CD4+ DC populations. On the other hand, the majority of thymocytes bound to DC strongly express the CD1, CD4, CD8 and CD2 antigens and weakly the CD3 antigen, indicating that they represent double-positive immature thymocytes. Uniform distribution of DC and thymocytes membrane antigens was confirmed with a backscattered SEM study. This morphological and immunolabeling TEM and SEM analysis demonstrates that human thymic DC may form associations with CD4+CD8+CD3weak thymocytes and raises questions about their physiological relationship. |
| Databáze: | OpenAIRE |
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