Woodsmoke Extracts Cross-Link Proteins and Induce Cornified Envelope Formation without Stimulating Keratinocyte Terminal Differentiation
| Autor: | Lo-Wei Lin, Robert H. Rice, Michael S. Denison |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Keratinocytes food.ingredient Cells air pollution Toxicology Cornified envelope transglutaminase 030207 dermatology & venereal diseases 03 medical and health sciences Liquid smoke 0302 clinical medicine food Viral envelope Tobacco Stratum corneum medicine oxidative stress 2.2 Factors relating to the physical environment Humans Aetiology Barrier function Cells Cultured Skin Smoke Cultured Corneocyte Transglutaminases Tobacco Smoke and Health liquid smoke Chemistry Cell Differentiation Pharmacology and Pharmaceutical Sciences Environmental Toxicology Cell biology 030104 developmental biology medicine.anatomical_structure aldehyde-induced protein adducts Epidermis Keratinocyte |
| Zdroj: | Toxicol Sci Toxicological sciences : an official journal of the Society of Toxicology, vol 183, iss 1 |
| ISSN: | 1096-0929 |
| Popis: | Air pollution poses a serious risk to human health. To help understand the contribution of smoke from wood burning to the harmfulness of air pollution toward the skin, we studied the effects of liquid smoke, aqueous extracts of wood smoke condensate, a commercially available food flavor additive, in cultured keratinocytes. We report that liquid smoke can react with and cross-link keratinocyte cellular proteins, leading to abnormal cross-linked envelope formation. Instead of inducing genes ordinarily involved in terminal differentiation, liquid smoke induced expression of genes associated with stress responses. When transglutaminase activity was inhibited, liquid smoke still promoted protein cross-linking and envelope formation in keratinocytes. This phenomenon likely results from oxidative stress and protein adducts from aldehydes as either preloading the cells with N-acetylcysteine or reducing the aldehyde content of liquid smoke decreased its ability to promote protein cross-linking and envelope formation. Finally, liquid smoke-induced envelopes were found to have elevated protein content, suggesting oxidative cross-linking and formation of protein adducts might impair barrier function by inducing abnormal incorporation of cellular proteins into envelopes. Since the cross-linked protein envelope provides structural stability to the stratum corneum and serves as a scaffold for the organization of the corneocyte lipid envelope (hydrophobic barrier to the environment), these findings provide new insight into the mechanism by which pro-oxidative air pollutants can impair epidermal function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|