Monoclonal Antibody-Defined Specific C Epitope of Brucella O-Polysaccharide Revisited
| Autor: | David R. Bundle, Julie Guiard, N. Vijaya Ganesh, Axel Cloeckaert, Michel S. Zygmunt |
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| Přispěvatelé: | Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, University of Alberta, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Microbiology (medical) Glycoconjugate medicine.drug_class Clinical Biochemistry Immunology Brucella Biology Monoclonal antibody Epitope Epitopes 03 medical and health sciences chemistry.chemical_compound Antigen Diagnostic Laboratory Immunology medicine Animals Humans Immunology and Allergy Tetrasaccharide 030304 developmental biology chemistry.chemical_classification 0303 health sciences Molecular Structure Perosamine 030306 microbiology Polysaccharides Bacterial Antibodies Monoclonal O Antigens Oligosaccharide biology.organism_classification Antibodies Bacterial [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Biochemistry |
| Zdroj: | Clinical and Vaccine Immunology Clinical and Vaccine Immunology, American Society for Microbiology, 2015, 22 (8), pp.979-982. ⟨10.1128/CVI.00225-15⟩ |
| ISSN: | 1556-6811 |
| DOI: | 10.1128/CVI.00225-15⟩ |
| Popis: | The C epitope of Brucella O-polysaccharide (O-PS) has so far lacked definitive structural identity. Revised structures for this antigen revealed a unique capping perosamine tetrasaccharide consisting of a sequence of 1,2:1,3:1,2 interresidue linkages. Here, using synthetic oligosaccharide glycoconjugates, the α-1,3 linkage of the O-PS is shown to be an integral structural requirement of this epitope. Although A-dominant strains possess only one or two copies of the capping tetrasaccharide, this creates a unique pentasaccharide antigenic determinant with the linkage sequence 1,2:1,3:1,2:1,2 that is always present in major pathogenic Brucella species. |
| Databáze: | OpenAIRE |
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